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Snake and Spider Toxins

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Avi Priel

    1. Faculty of Medicine, School of Pharmacy, Institute for Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2068)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Overview

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Snake- and Spider-Venom-Derived Toxins as Lead Compounds for Drug Development

      • Philip Lazarovici
      Pages 3-26

    3. Analytics for Bioactivity Profiling of Complex Mixtures with a Focus on Venoms

      • Marija Mladic, Wilfried M. A. Niessen, Govert W. Somsen, Jeroen Kool
      Pages 27-49

  3. Toxins Purification and Production

    1. Front Matter

      Pages 51-51
      Download chapter PDF

    2. Venom Collection from Spiders and Snakes: Voluntary and Involuntary Extractions (“Milking”) and Venom Gland Extractions

      • William K. Hayes, Gerad A. Fox, David R. Nelsen
      Pages 53-71

    3. Production and Purification of Recombinant Toxins

      • Matan Geron
      Pages 73-84

  4. Toxins Characterization

    1. Front Matter

      Pages 85-85
      Download chapter PDF

    2. RNA-Sequencing of Snake Venom Glands

      • Khin Than Yee, Olga Vasieva, Ponlapat Rojnuckarin
      Pages 87-96

    3. Exploring Toxin Evolution: Venom Protein Transcript Sequencing and Transcriptome-Guided High-Throughput Proteomics

      • Cassandra M. Modahl, Jordi Durban, Stephen P. Mackessy
      Pages 97-127

    4. Three-Dimensional Structure Determination of Peptides Using Solution Nuclear Magnetic Resonance Spectroscopy

      • Christina I. Schroeder, K. Johan Rosengren
      Pages 129-162

    5. Determining the Structures of the Snake and Spider Toxins by X-Rays

      • Anwar Ullah, Rehana Masood, Zafar Hayat, Ahmed Hafeez
      Pages 163-172

    6. MALDI-TOF Mass Spectrometric Profiling of Spider Venoms

      • Ondrej Šedo, Stano Pekár, Zbyněk Zdráhal
      Pages 173-181

  5. Characterization of Toxins Function

    1. Front Matter

      Pages 183-183
      Download chapter PDF

    2. Methods for Evaluation of a Snake Venom-Derived Disintegrin in Animal Models of Human Cancer

      • Stephen D. Swenson, Catalina Silva-Hirschberg, Francis S. Markland
      Pages 185-204

    3. Cell-Based Adhesion Assays for Isolation of Snake Venom’s Integrin Antagonists

      • Philip Lazarovici, Cezary Marcinkiewicz, Peter I. Lelkes
      Pages 205-223

    4. Using C. elegans to Study the Effects of Toxins in Sensory Ion Channels In Vivo

      • Valeria Vásquez
      Pages 225-238

    5. Measurements of Cell Death Induced by Snake and Spider’s Venoms and Derived Toxins

      • Yossi Maatuf, Avi Priel, Philip Lazarovici
      Pages 239-268

    6. Using Toxins in Brain Slice Recordings

      • Alexey Bingor, Rami Yaka
      Pages 269-274

    7. High-Throughput Calcium Imaging Screen of Toxins’ Function in Dissociated Sensory Neurons

      • Yossi Maatuf, Avi Priel
      Pages 275-282
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Keywords

About this book

This volume explores techniques and protocols, across various biological disciplines, used to study snake and spider peptide toxins. The chapters in this book are organized into four parts and cover topics such as the use of toxins in drug development; analysis of bioactivity of complex mixtures like venom; extraction of venom glands and the production of toxins; characterization of toxins from the RNA level to the peptide structure; and the determination of the toxin’s biological function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and thorough, Snake and Spider Toxins: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field. 


Editors and Affiliations

  • Faculty of Medicine, School of Pharmacy, Institute for Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel

    Avi Priel

Bibliographic Information

  • Book Title: Snake and Spider Toxins

  • Book Subtitle: Methods and Protocols

  • Editors: Avi Priel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9845-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9844-9Published: 02 October 2019

  • Softcover ISBN: 978-1-4939-9847-0Published: 02 October 2020

  • eBook ISBN: 978-1-4939-9845-6Published: 02 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 293

  • Number of Illustrations: 14 b/w illustrations, 32 illustrations in colour

  • Topics: Pharmacology/Toxicology

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