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Diabetic Nephropathy

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Luigi Gnudi

    1. Faculty of Life Sciences & Medicine School of Cardiovascular Medicine & Sciences, British Heart Foundation Centre of Research Excellence, King’s College London, London, UK

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  2. David A. Long

    1. Developmental Biology and Cancer Programme, UCL Great Ormond Street Institute of Child Health, University College London, London, UK

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2067)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. Diabetic Nephropathy: An Overview

      • Manpreet K. Sagoo, Luigi Gnudi
      Pages 3-7

  3. Experimental Models to Study Diabetic Nephropathy

    1. Front Matter

      Pages 9-9
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    2. Modeling Podocyte Biology Using Drosophila Nephrocytes

      • Paul S. Hartley, Richard J. Coward
      Pages 11-24

    3. A Technique for Studying Glomerular Filtration Integrity in the Zebrafish Pronephros

      • Maria Kolatsi-Joannou, Daniel Osborn
      Pages 25-39

    4. Modeling Human Diabetic Kidney Disease by Combining Hyperglycemia and Hypertension in a Transgenic Rodent Model

      • Carolynn Cairns, Bryan Conway
      Pages 41-52

    5. Isolating and Culturing Mouse Podocyte Cells to Study Diabetic Nephropathy

      • Elisavet Vasilopoulou
      Pages 53-59

  4. Techniques to Image the Diabetic Kidney

    1. Front Matter

      Pages 61-61
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    2. Histological Examination of the Diabetic Kidney

      • Camillo Carrara, Mauro Abbate, Sara Conti, Daniela Rottoli, Paola Rizzo, Gianfranco Marchetti
      Pages 63-87

    3. Sample Preparation and Stereological Methods for the Study of Glomerular Ultrastructure Using Electron Microscopy

      • Kathryn E. White
      Pages 89-102

    4. Tissue Clearing and Deep Imaging of the Kidney Using Confocal and Two-Photon Microscopy

      • Daniyal J. Jafree, David A. Long, Peter J. Scambler, Dale Moulding
      Pages 103-126

  5. Assessing the Function and Pathophysiological Properties of the Diabetic Kidney

    1. Front Matter

      Pages 127-127
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    2. Transcutaneous Measurement of Glomerular Filtration Rate in Rodents

      • Cristina Daniele, Daniela Nardozi, Angelo Torelli, Arif ul Maula Khan, Norbert Gretz
      Pages 129-137

    3. An In Vitro Method to Analyze Glucose Uptake in Podocytes

      • Abigail C. Lay, Richard J. Coward
      Pages 139-143

    4. Glomerular Endothelial Cells: Assessment of Barrier Properties In Vitro

      • Raina D. Ramnath, Simon C. Satchell
      Pages 145-151

    5. Methods to Detect Endoplasmic Reticulum Stress and Apoptosis in Diabetic Nephropathy

      • Khurrum Shahzad, Sanchita Ghosh, Akash Mathew, Berend Isermann
      Pages 153-173

    6. Isolating Urinary Extracellular Vesicles as Biomarkers for Diabetic Disease

      • Karina Barreiro, Tobias B. Huber, Harry Holthofer
      Pages 175-188

    7. Examining Cell-Cell Interactions in the Kidney Using AFM Single-Cell Force Spectroscopy

      • Eleftherios Siamantouras, Claire E. Hills, Kuo-Kang Liu, Paul E. Squires
      Pages 189-201

  6. Transcriptome, Metabolic, and Proteomic Profiling of the Diabetic Kidney

    1. Front Matter

      Pages 203-203
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Keywords

About this book

This book provides a toolkit of novel research approaches for investigators to study diabetic nephropathy, including critical experimental models from the fly to the fish, cells in culture, and in vivo mammalian approaches. The collection also explores powerful techniques to image the kidney, such as traditional histological techniques as well as electron, confocal, and two-photon microscopy, pathophysiology of the diabetic kidney, and gene editing and regenerative medicine. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Diabetic Nephropathy: Methods and Protocols seeks to foster new research directions and inspire ideas to enhance our understanding of diabetic nephropathy and todevelop treatments for this condition.

Editors and Affiliations

  • Faculty of Life Sciences & Medicine School of Cardiovascular Medicine & Sciences, British Heart Foundation Centre of Research Excellence, King’s College London, London, UK

    Luigi Gnudi

  • Developmental Biology and Cancer Programme, UCL Great Ormond Street Institute of Child Health, University College London, London, UK

    David A. Long

Bibliographic Information

  • Book Title: Diabetic Nephropathy

  • Book Subtitle: Methods and Protocols

  • Editors: Luigi Gnudi, David A. Long

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9841-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9840-1Published: 08 November 2019

  • Softcover ISBN: 978-1-4939-9843-2Published: 08 November 2020

  • eBook ISBN: 978-1-4939-9841-8Published: 07 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 342

  • Number of Illustrations: 14 b/w illustrations, 95 illustrations in colour

  • Topics: Molecular Medicine, Nephrology, Diabetes

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