Methods in Molecular Biology

Transgenic Mouse

Methods and Protocols

Editors: Larson, Melissa (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-9837-1
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About this book

This volume provides readers with a historical foundation in standard techniques and a comprehensive update on the latest methods used in making gene-modified mice. The chapters in this book cover topics such as pronuclear microinjection in one-cell embryos; embryo transfer surgery; nuclear transfer and cloning; blastocyst microinjection; and cryobanking and recovery of genetically modified mice. Importantly, there are chapters devoted to the latest application of CRISPR technology, as well as the establishment of induced pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Transgenic Mouse: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this evolving field. 


Table of contents (18 chapters)

Table of contents (18 chapters)
  • The History of Transgenesis

    Pages 1-26

    Saunders, Thomas L.

  • Pronuclear Microinjection of One-Cell Embryos

    Pages 27-33

    Larson, Melissa A.

  • Integrase-Mediated Targeted Transgenics Through Pronuclear Microinjection

    Pages 35-46

    Chen-Tsai, Ruby Yanru

  • In Vivo Validation of CRISPR Reagents in Preimplantation Mouse Embryos

    Pages 47-57

    Larson, Melissa A. (et al.)

  • Targeted Mutations in the Mouse via Embryonic Stem Cells

    Pages 59-82

    Gertsenstein, Marina (et al.)

Buy this book

eBook n/a
  • ISBN 978-1-4939-9837-1
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
Hardcover n/a
  • ISBN 978-1-4939-9836-4
  • Free shipping for individuals worldwide
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Bibliographic Information

Bibliographic Information
Book Title
Transgenic Mouse
Book Subtitle
Methods and Protocols
Editors
  • Melissa Larson
Series Title
Methods in Molecular Biology
Series Volume
2066
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9837-1
DOI
10.1007/978-1-4939-9837-1
Hardcover ISBN
978-1-4939-9836-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 236
Number of Illustrations
18 b/w illustrations, 27 illustrations in colour
Topics