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Methods in Molecular Biology

Quantitative Real-Time PCR

Methods and Protocols

Editors: Biassoni, Roberto, Raso, Alessandro (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9833-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9832-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • A Quarter Century of PCR-Applied Techniques and Their Still-Increasing Fields of Use

    Pages 1-4

    Raso, Alessandro (et al.)

  • Parameters for Successful PCR Primer Design

    Pages 5-22

    Bustin, Stephen A. (et al.)

  • MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing

    Pages 23-38

    Mussack, Veronika (et al.)

  • Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A) Tailing and 5′ Adaptor Ligation

    Pages 39-54

    Vautrot, Valentin (et al.)

  • A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PC R

    Pages 55-64

    Biswas, Priscilla (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9833-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9832-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Quantitative Real-Time PCR
Book Subtitle
Methods and Protocols
Editors
  • Roberto Biassoni
  • Alessandro Raso
Series Title
Methods in Molecular Biology
Series Volume
2065
Copyright
2020
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9833-3
DOI
10.1007/978-1-4939-9833-3
Hardcover ISBN
978-1-4939-9832-6
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIII, 235
Number of Illustrations
15 b/w illustrations, 55 illustrations in colour
Topics