Methods in Molecular Biology

Single Cell Metabolism

Methods and Protocols

Editors: Shrestha, Bindesh (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-9831-9
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Hardcover $199.99
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  • ISBN 978-1-4939-9829-6
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About this book

This volume explores the latest techniques and workflow for the analysis of single cells metabolism. The chapters in this book cover topics such as the development of mass spectrometry-based single cell approaches, Pico-ESI-MS for single-cell metabolomics analysis; laser capture microdissection; ambient single cell metabolite profile (DESI and LAESI); and MALDI-MS methodology, quantum dots for quantitative cytology to study metabolic heterogeneity of single cells. Written in the highly successful Methods in Molecular Biology series format, the chapters consist of  introductions to the topic, lists of the necessary materials and reagents, step-by-step guidelines, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Single Cell Metabolism: Methods and Protocols is a valuable resource for any researcher and scientist interested in learning more about this field. 

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Single-Cell Metabolomics by Mass Spectrometry

    Pages 1-8

    Shrestha, Bindesh

  • Identification of Metabolites in Single Cells by Ion Mobility Separation and Mass Spectrometry

    Pages 9-18

    Zhang, Linwen (et al.)

  • Analysis of Lipids in Single Cells and Organelles Using Nanomanipulation-Coupled Mass Spectrometry

    Pages 19-30

    Phelps, Mandy S. (et al.)

  • Development of Pico-ESI-MS for Single-Cell Metabolomics Analysis

    Pages 31-59

    Wei, Zhenwei (et al.)

  • Single-Probe Mass Spectrometry Analysis of Metabolites in Single Cells

    Pages 61-71

    Pan, Ning (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9831-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9829-6
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Single Cell Metabolism
Book Subtitle
Methods and Protocols
Editors
  • Bindesh Shrestha
Series Title
Methods in Molecular Biology
Series Volume
2064
Copyright
2020
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9831-9
DOI
10.1007/978-1-4939-9831-9
Hardcover ISBN
978-1-4939-9829-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 226
Number of Illustrations
10 b/w illustrations, 64 illustrations in colour
Topics