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Methods in Molecular Biology

The Eukaryotic RNA Exosome

Methods and Protocols

Editors: LaCava, John, Vaňáčová, Štěpánka (Eds.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts
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About this book

This volume provides a cross-section of RNA exosome research protocols, applied to a diversity of model organisms. Chapters guide readers through methods that e.g. delineate eukaryotic exosomes’ origins in prokaryotes, probe its RNA substrates, adapter complexes and macromolecular interaction of networks, and establish critical structural-function relationships. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, The Eukaryotic RNA Exosome: Methods and Protocols aims to ensure successful results in the further study of this vital field.


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Table of contents (24 chapters)

Table of contents (24 chapters)
  • The RNA Exosome and Human Disease

    Pages 3-33

    Fasken, Milo B. (et al.)

  • The Bacterial Counterparts of the Eukaryotic Exosome: An Evolutionary Perspective

    Pages 37-46

    Viegas, Sandra C. (et al.)

  • In Vitro Characterization of the Prokaryotic Counterparts of the Exosome Complex

    Pages 47-61

    Matos, Rute G. (et al.)

  • Enzymatic Analysis of Reconstituted Archaeal Exosomes

    Pages 63-79

    Evguenieva-Hackenberg, Elena (et al.)

  • Protocols for Northern Analysis of Exosome Substrates and Other Noncoding RNAs

    Pages 83-103

    Cruz, Cristina (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-4939-9822-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9821-0
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The Eukaryotic RNA Exosome
Book Subtitle
Methods and Protocols
Editors
  • John LaCava
  • Štěpánka Vaňáčová
Series Title
Methods in Molecular Biology
Series Volume
2062
Copyright
2020
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9822-7
DOI
10.1007/978-1-4939-9822-7
Hardcover ISBN
978-1-4939-9821-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 517
Number of Illustrations
27 b/w illustrations, 52 illustrations in colour
Topics

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