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Plant Meiosis

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2061)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
  2. Cytological and Imaging Approaches to Study Arabidopsis Meiosis

    1. Front Matter

      Pages 1-1
    2. A Practical Guide to Live-Cell Imaging of Meiosis in Arabidopsis

      • Maria Ada Prusicki, Yuki Hamamura, Arp Schnittger
      Pages 3-12
    3. Whole-Mount Immunolocalization Procedure for Plant Female Meiocytes

      • Santiago Valentín Galvan Gordillo, Rocio Escobar-Guzman, Daniel Rodriguez-Leal, Jean-Philippe Vielle-Calzada, Arnaud Ronceret
      Pages 13-24
    4. Targeted Analysis of Chromatin Events (TACE)

      • Jason Sims, Changbin Chen, Peter Schlögelhofer, Marie-Therese Kurzbauer
      Pages 47-58
    5. Whole-Mount Immuno-FISH on Arabidopsis Meiocytes (WhoMI-FISH)

      • Jason Sims, Jihed Chouaref, Peter Schlögelhofer
      Pages 59-66
  3. Cytological and Imaging Approaches to Study Meiosis in Other Species

    1. Front Matter

      Pages 67-67
    2. Preparing Maize Synaptonemal Complex Spreads and Sequential Immunofluorescence and Fluorescence In Situ Hybridization

      • Stephen M. Stack, Lindsay A. Shearer, Leslie D. Lohmiller, Lorinda K. Anderson
      Pages 79-115
    3. Cytological Techniques to Study Cytomixis in Plant Male Meiosis

      • Sergey R. Mursalimov, Yuriy V. Sidorchuk, Elena V. Deineko
      Pages 117-129
    4. Analysis of Meiosis in Nonmodel Tropical Plants: The Case of Carica papaya Linn

      • José Mora-Calderón, Kalani Scott-Moraga, Pablo Bolaños-Villegas
      Pages 131-139
    5. Following the Formation of Synaptonemal Complex Formation in Wheat and Barley by High-Resolution Microscopy

      • Benoit Darrier, Mikel Arrieta, Sybille U. Mittmann, Pierre Sourdille, Luke Ramsay, Robbie Waugh et al.
      Pages 207-215
  4. Molecular and Biochemical Approaches to Study Meiosis

    1. Front Matter

      Pages 217-217
    2. Chromatin Immunoprecipitation of Meiotically Expressed Proteins from Arabidopsis thaliana Flowers

      • Christophe Lambing, Kyuha Choi, Alexander R. Blackwell, Ian R. Henderson
      Pages 219-236

About this book

This volume looks at the latest techniques used by the meiosis research community to study plant meiosis. The chapters in this book are organized into four parts: Part One discusses cytological and imaging approaches to study meiosis and chromosome dynamics in Arabidopsis (in both diploid and polyploid backgrounds); Part Two talks about using cytological methods to study meiosis in other plant species; Part Three focuses on molecular and biochemical approaches to look at plant meiosis; and Part Four explores further procedures and experiments that are helpful in learning more about plant meiosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Plant Meiosis: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in learning more about this developing field. 



Editors and Affiliations

  • Departamento de Genética, Fisiología y Microbiología, Facultad de Biología, Universidad Complutense de Madrid, Madrid, Spain

    Mónica Pradillo

  • Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) OT Gatersleben, Stadt Seeland, Germany

    Stefan Heckmann

Bibliographic Information

  • Book Title: Plant Meiosis

  • Book Subtitle: Methods and Protocols

  • Editors: Mónica Pradillo, Stefan Heckmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9818-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9817-3Published: 04 October 2019

  • Softcover ISBN: 978-1-4939-9820-3Published: 04 October 2020

  • eBook ISBN: 978-1-4939-9818-0Published: 03 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 405

  • Number of Illustrations: 10 b/w illustrations, 66 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access