Herpes Simplex Virus
Methods and Protocols
Editors: Diefenbach, Russell, Fraefel, Cornel (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV.
- Table of contents (27 chapters)
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Tour de Herpes: Cycling Through the Life and Biology of HSV-1
Pages 1-30
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Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology
Pages 31-56
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Herpes Simplex Virus Growth, Preparation, and Assay
Pages 57-72
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Engineering HSV-1 Vectors for Gene Therapy
Pages 73-90
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Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors
Pages 91-109
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Table of contents (27 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Herpes Simplex Virus
- Book Subtitle
- Methods and Protocols
- Editors
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- Russell Diefenbach
- Cornel Fraefel
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2060
- Copyright
- 2020
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9814-2
- DOI
- 10.1007/978-1-4939-9814-2
- Hardcover ISBN
- 978-1-4939-9813-5
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XIV, 457
- Number of Illustrations
- 15 b/w illustrations, 45 illustrations in colour
- Topics
