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Methods in Molecular Biology

Herpes Simplex Virus

Methods and Protocols

Editors: Diefenbach, Russell, Fraefel, Cornel (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9814-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9813-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV. 

Table of contents (27 chapters)

Table of contents (27 chapters)
  • Tour de Herpes: Cycling Through the Life and Biology of HSV-1

    Pages 1-30

    Denes, Christopher E. (et al.)

  • Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology

    Pages 31-56

    Sandgren, Kerrie J. (et al.)

  • Herpes Simplex Virus Growth, Preparation, and Assay

    Pages 57-72

    Sutter, Sereina O. (et al.)

  • Engineering HSV-1 Vectors for Gene Therapy

    Pages 73-90

    Goins, William F. (et al.)

  • Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors

    Pages 91-109

    Fraefel, Cornel (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9814-2
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9813-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Herpes Simplex Virus
Book Subtitle
Methods and Protocols
Editors
  • Russell Diefenbach
  • Cornel Fraefel
Series Title
Methods in Molecular Biology
Series Volume
2060
Copyright
2020
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9814-2
DOI
10.1007/978-1-4939-9814-2
Hardcover ISBN
978-1-4939-9813-5
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIV, 457
Number of Illustrations
15 b/w illustrations, 45 illustrations in colour
Topics