Herpes Simplex Virus

1. Front Matter

   Pages i-xiv

   PDF

2. Tour de Herpes: Cycling Through the Life and Biology of HSV-1

   • Christopher E. Denes, Roger D. Everett, Russell J. Diefenbach

   Pages 1-30

3. Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology

   • Kerrie J. Sandgren, Naomi R. Truong, Jacinta B. Smith, Kirstie Bertram, Anthony L. Cunningham

   Pages 31-56

4. Herpes Simplex Virus Growth, Preparation, and Assay

   • Sereina O. Sutter, Peggy Marconi, Anita F. Meier

   Pages 57-72

5. Engineering HSV-1 Vectors for Gene Therapy

   • William F. Goins, Shaohua Huang, Bonnie Hall, Marco Marzulli, Justus B. Cohen, Joseph C. Glorioso

   Pages 73-90

6. Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors

   • Cornel Fraefel, Alberto L. Epstein

   Pages 91-109

7. HSV-1 Amplicon Vectors as Genetic Vaccines

   • Anita F. Meier, Andrea S. Laimbacher

   Pages 111-130

8. oHSV Genome Editing by Means of galK Recombineering

   • Laura Menotti, Valerio Leoni, Valentina Gatta, Biljana Petrovic, Andrea Vannini, Simona Pepe et al.

   Pages 131-151

9. Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein

   • Andrea Vannini, Biljana Petrovic, Valentina Gatta, Valerio Leoni, Simona Pepe, Laura Menotti et al.

   Pages 153-168

10. CRISPR/Cas9-Based Genome Editing of HSV

    • Thilaga Velusamy, Anjali Gowripalan, David C. Tscharke

    Pages 169-183

11. Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescence In Situ Hybridization (FISH)

    • Camille Cohen, Armelle Corpet, Mohamed Ali Maroui, Franceline Juillard, Patrick Lomonte

    Pages 185-197

12. Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for Use in High-Throughput Sequencing

    • Mackenzie M. Shipley, Molly M. Rathbun, Moriah L. Szpara

    Pages 199-217

13. HSV Mutant Generation and Dual Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo

    • Nancy M. Sawtell, Richard L. Thompson

    Pages 219-239

14. Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals

    • Andreas Sauerbrei, Kathrin Bohn-Wippert

    Pages 241-261

15. Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation

    • Hui-Lan Hu, Kalanghad Puthankalam Srinivas, Ian Mohr, Tony T. Huang, Angus C. Wilson

    Pages 263-277

16. Characterization of Extracellular HSV-1 Virions by Proteomics

    • Roger Lippé

    Pages 279-288

17. Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry

    • Bita Khadivjam, Nabil El Bilali, Roger Lippé

    Pages 289-303

18. Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells

    • Raquel Bello-Morales, José Antonio López-Guerrero

    Pages 305-317

19. Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot

    • Tri Komala Sari, Katrina A. Gianopulos, Anthony V. Nicola

    Pages 319-326

20. BioID Combined with Mass Spectrometry to Study Herpesvirus Protein–Protein Interaction Networks

    • Mujeeb R. Cheerathodi, David G. Meckes Jr.

    Pages 327-341

This second edition volume expands on the previous edition with a discussion of new and updated methods used to study the Herpes Simplex Virus (HSV), along with a look at the latest developing technologies such as next generation sequencing, CRISPR/Cas9 engineering, and the use of BioID to identify protein-protein interactions. Chapters cover topics such as the biology, life cycle, and current state of antiviral and vaccine development for HSV-1; protocols on growing viruses in cell culture and manipulating viral DNA; design and application of HSV-1 vectors for cancer- and gene-therapy; and structural analyses, microscopy, proteomics, and testing of antivirals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 
Cutting-edge and comprehensive, Herpes Simplex Virus: Methods and Protocols, Second Edition is a valuable resource for immunologists, and molecular and cell biologists. This book will also be useful for researchers who wish to initiate molecular and/or cellular-based approaches to study HSV. 

• Nucleocapsid DNA
• Bacterial Artificial Chromosomes (BACs)
• Latent/Lytic Cycles In Vivo
• Cryo-EM
• CRISPR-Cas9
• infectious diseases

• Department of Biomedical Sciences, Macquarie University, Sydney, Australia

  Russell J. Diefenbach

• Institute of Virology, University of Zurich, Zürich, Switzerland

  Cornel Fraefel

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