Methods in Molecular Biology

Nitrogen Metabolism in Plants

Methods and Protocols

Editors: Gupta, Kapuganti Jagadis (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field.


Table of contents (14 chapters)

Table of contents (14 chapters)
  • An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants

    Pages 1-13

    Kishorekumar, Reddy (et al.)

  • Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues

    Pages 15-26

    Wany, Aakanksha (et al.)

  • Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves Under Different Conditions

    Pages 27-35

    Bulle, Mallesham (et al.)

  • Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples

    Pages 37-43

    Mioto, Paulo T. (et al.)

  • Measurement of S-Nitrosoglutathione Reductase Activity in Plants

    Pages 45-59

    Janků, Martina (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9790-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-1-4939-9789-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Nitrogen Metabolism in Plants
Book Subtitle
Methods and Protocols
Editors
  • Kapuganti Jagadis Gupta
Series Title
Methods in Molecular Biology
Series Volume
2057
Copyright
2020
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9790-9
DOI
10.1007/978-1-4939-9790-9
Hardcover ISBN
978-1-4939-9789-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 178
Number of Illustrations
9 b/w illustrations, 32 illustrations in colour
Topics