- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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- About this book
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This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats. Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.
- Table of contents (17 chapters)
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Experimenting with Trinucleotide Repeats: Facts and Technical Issues
Pages 1-10
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Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions
Pages 11-23
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Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae
Pages 25-68
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Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis
Pages 69-81
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Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae
Pages 83-101
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Table of contents (17 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Trinucleotide Repeats
- Book Subtitle
- Methods and Protocols
- Editors
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- Guy-Franck Richard
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2056
- Copyright
- 2020
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9784-8
- DOI
- 10.1007/978-1-4939-9784-8
- Hardcover ISBN
- 978-1-4939-9783-1
- Softcover ISBN
- 978-1-4939-9786-2
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XI, 286
- Number of Illustrations
- 12 b/w illustrations, 53 illustrations in colour
- Topics
