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Methods in Molecular Biology

Trinucleotide Repeats

Methods and Protocols

Editors: Richard, Guy-Franck (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $14.99
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  • ISBN 978-1-4939-9784-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9783-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This detailed book contains techniques to explore the unusual properties of the peculiar microsatellites known as trinucleotide repeats.  Beginning with technical challenges raised by secondary structure-forming repeats and their propensity to contract and expand over time, the book continues with genetic screens in Saccharomyces cerevisiae, experimental systems to study trinucleotide repeat instability in human cells, dedicated experimental systems in appropriate cell types, as well as gene therapy approaches using the CRISPR-Cas family of endonucleases.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Trinucleotide Repeats: Methods and Protocols serves as a valuable aid to experts and newcomers alike who seek to investigate this fascinating and ever-expanding field of study.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Experimenting with Trinucleotide Repeats: Facts and Technical Issues

    Pages 1-10

    Richard, Guy-Franck

  • Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions

    Pages 11-23

    Tomé, Stéphanie (et al.)

  • Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae

    Pages 25-68

    Williams, Gregory M. (et al.)

  • Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis

    Pages 69-81

    Viterbo, David (et al.)

  • Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae

    Pages 83-101

    Polleys, Erica J. (et al.)

Buy this book

eBook $14.99
price for USA in USD (gross)
  • ISBN 978-1-4939-9784-8
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9783-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Trinucleotide Repeats
Book Subtitle
Methods and Protocols
Editors
  • Guy-Franck Richard
Series Title
Methods in Molecular Biology
Series Volume
2056
Copyright
2020
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9784-8
DOI
10.1007/978-1-4939-9784-8
Hardcover ISBN
978-1-4939-9783-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 286
Number of Illustrations
12 b/w illustrations, 53 illustrations in colour
Topics