Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail methods and reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2050)
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Table of contents (21 protocols)
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Front Matter
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Design and Fabrication of Micro-/Nano-Electroporation
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Front Matter
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Micro-/Nano-Electroporation for In Vitro Delivery
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Front Matter
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Advanced In Vivo Electroporation
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Front Matter
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Microorganism Electroporation
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Front Matter
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About this book
This third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice, Third Edition aims to be an invaluable resource for investigators both in and outside of this field.
Editors and Affiliations
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MD Anderson Cancer Center, The University of Texas, Houston, USA
Shulin Li
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School of Biological Science and Medical Engineering, Beihang University, Beijing, China, Institute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University,, Beijing, China
Lingqian Chang
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Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France
Justin Teissie
Bibliographic Information
Book Title: Electroporation Protocols
Book Subtitle: Microorganism, Mammalian System, and Nanodevice
Editors: Shulin Li, Lingqian Chang, Justin Teissie
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9740-4
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-4939-9739-8Published: 30 August 2019
Softcover ISBN: 978-1-4939-9742-8Published: 30 August 2020
eBook ISBN: 978-1-4939-9740-4Published: 29 August 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 3
Number of Pages: XI, 196
Number of Illustrations: 7 b/w illustrations, 31 illustrations in colour
Topics: Gene Therapy