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Methods in Molecular Biology

Stable Isotope Probing

Methods and Protocols

Editors: Dumont, Marc G., Hernández García, Marcela (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9721-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9720-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.

Table of contents (18 chapters)

Table of contents (18 chapters)
  • Experimental Setup and Data Analysis Considerations for DNA- and RNA-SIP Experiments in the Omics Era

    Pages 1-15

    Angel, Roey

  • DNA-Based Stable Isotope Probing

    Pages 17-29

    Jia, Zhongjun (et al.)

  • RNA Stable Isotope Probing (RNA-SIP)

    Pages 31-44

    Ghori, Noor-Ul-Huda (et al.)

  • Stable Isotope Probing of Microbial Phospholipid Fatty Acids in Environmental Samples

    Pages 45-55

    Watzinger, Andrea (et al.)

  • SIP-Metaproteomics: Linking Microbial Taxonomy, Function, and Activity

    Pages 57-69

    Taubert, Martin

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9721-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9720-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Stable Isotope Probing
Book Subtitle
Methods and Protocols
Editors
  • Marc G. Dumont
  • Marcela Hernández García
Series Title
Methods in Molecular Biology
Series Volume
2046
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9721-3
DOI
10.1007/978-1-4939-9721-3
Hardcover ISBN
978-1-4939-9720-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 247
Number of Illustrations
8 b/w illustrations, 35 illustrations in colour
Topics