Cerebrospinal Fluid (CSF) Proteomics
Methods and Protocols
Editors: Santamaría, Enrique, Fernández-Irigoyen, Joaquín (Eds.)
Free Preview- Includes cutting-edge methods and protocols
- provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume focuses on protein analysis, including a wide range of the use of mass spectrometry and other protein methods within neurobiological disciplines. Chapters cover topics such as cerebrospinal fluid (CSF) processing and biobanking; label-free quantitative proteomics; SWATH; top-down proteomics; and experimental strategies based on other –omics applied to CSF metabolome, lipidome, and microRNAome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols is a valuable resource for graduate students and post-doctoral fellows interested in learning more about CSF proteotyping. It is also useful to established researchers seeking further insight into this growing field.
- Table of contents (26 chapters)
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The Human Brain Proteome Project: Biological and Technological Challenges
Pages 3-23
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Guidelines for CSF Processing and Biobanking: Impact on the Identification and Development of Optimal CSF Protein Biomarkers
Pages 27-50
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Functional Analyses of Embryonic Cerebrospinal Fluid Proteins
Pages 51-60
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CSF Sample Preparation for Data-Independent Acquisition
Pages 61-67
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Sample Fractionation Techniques for CSF Peptide Spectral Library Generation
Pages 69-77
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Table of contents (26 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Cerebrospinal Fluid (CSF) Proteomics
- Book Subtitle
- Methods and Protocols
- Editors
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- Enrique Santamaría
- Joaquín Fernández-Irigoyen
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2044
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9706-0
- DOI
- 10.1007/978-1-4939-9706-0
- Hardcover ISBN
- 978-1-4939-9705-3
- Softcover ISBN
- 978-1-4939-9708-4
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XV, 557
- Number of Illustrations
- 3 b/w illustrations, 164 illustrations in colour
- Topics
