- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains keynotes and implementation advice from the experts
Buy this book
- About this book
-
This volume covers the latest techniques and strategies used in multi-photon excitation (MPE) microscopy. Chapters in this book cover the fundamentals of MPE microscopy as applied to both in vitro and in vivo experimental systems; information on how to combine MPE microscopy with targeted electrophysiological recordings, calcium imaging, and transmembrane voltage imaging; methods to investigate cellular and large-scale neural morphology; signaling in astrocytes; and ways to use MPE microscopy to study the retina. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Comprehensive and thorough, Multiphoton Microscopy is a valuable resource for both expert and novice researchers interested in expanding their knowledge and research in this rapidly developing field.
- Table of contents (14 chapters)
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Building a Two-Photon Microscope Is Easy
Pages 1-16
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Using Multiphoton Imaging for Targeted Electrophysiological Recording and Live Cell Imaging of Fluorescently Labeled Neurons from Isolated Retinas
Pages 17-31
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Two-Photon Neurotransmitter Uncaging for the Study of Dendritic Integration
Pages 33-64
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Two-Photon Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines
Pages 65-85
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Two-Photon Fluorescence Imaging of Visually Evoked Glutamate Release Using iGluSnFR in the Mouse Visual System
Pages 87-109
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Table of contents (14 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Multiphoton Microscopy
- Editors
-
- Espen Hartveit
- Series Title
- Neuromethods
- Series Volume
- 148
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9702-2
- DOI
- 10.1007/978-1-4939-9702-2
- Hardcover ISBN
- 978-1-4939-9701-5
- Series ISSN
- 0893-2336
- Edition Number
- 1
- Number of Pages
- XII, 364
- Number of Illustrations
- 4 b/w illustrations, 82 illustrations in colour
- Topics
