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Methods in Molecular Biology

Chlamydia trachomatis

Methods and Protocols

Editors: Brown, Amanda Claire (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9694-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9693-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.

Table of contents (18 chapters)

Table of contents (18 chapters)
  • Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion Based Amplification (mSIBA)

    Pages 1-9

    Eboigbodin, Kevin E. (et al.)

  • Point-of-Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue

    Pages 11-17

    Somboonna, Naraporn (et al.)

  • Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae

    Pages 19-25

    Eboigbodin, Kevin E.

  • Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay

    Pages 27-32

    Eboigbodin, Kevin E.

  • Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth

    Pages 33-43

    Meštrović, Tomislav (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9694-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9693-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Chlamydia trachomatis
Book Subtitle
Methods and Protocols
Editors
  • Amanda Claire Brown
Series Title
Methods in Molecular Biology
Series Volume
2042
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9694-0
DOI
10.1007/978-1-4939-9694-0
Hardcover ISBN
978-1-4939-9693-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 289
Number of Illustrations
7 b/w illustrations, 26 illustrations in colour
Topics