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Chlamydia trachomatis

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Amanda Claire Brown

    1. Department of Microbiology and Immunology, Vet School, Cornell University, Ithaca, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2042)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion Based Amplification (mSIBA)

    • Kevin E. Eboigbodin, Mark Hoser
    Pages 1-9

  3. Point-of-Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue

    • Naraporn Somboonna, Ilada Choopara
    Pages 11-17

  4. Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae

    • Kevin E. Eboigbodin
    Pages 19-25

  5. Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay

    • Kevin E. Eboigbodin
    Pages 27-32

  6. Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth

    • Tomislav Meštrović, Dezső P. Virok, Sunčanica Ljubin-Sternak, Tímea Raffai, Katalin Burián, Jasmina Vraneš
    Pages 33-43

  7. Whole-Genome Sequencing of Chlamydia trachomatis Directly from Human Samples

    • Amanda Claire Brown, Mette T. Christiansen
    Pages 45-67

  8. Multilocus Sequence Typing (MLST) of Chlamydiales

    • Martina Jelocnik, Adam Polkinghorne, Yvonne Pannekoek
    Pages 69-86

  9. Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, A Double-Locus Genotyping Method

    • Deborah C. Holt, Patiyan Andersson, Cameron Buckley, David M. Whiley, Philip M. Giffard
    Pages 87-122

  10. Dual RNA-Seq of Chlamydia and Host Cells

    • James W. Marsh, Regan J. Hayward, Amol Shetty, Anup Mahurkar, Michael S. Humphrys, Garry S. A. Myers
    Pages 123-135

  11. Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection

    • Oleg V. Podgorny, Nadezhda F. Polina, Vassili N. Lazarev
    Pages 137-150

  12. Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions

    • Katerina Wolf, Mostafa Rahnama, Kenneth A. Fields
    Pages 151-164

  13. Mutagenesis of Chlamydia trachomatis Using TargeTron

    • Mary M. Weber, Robert Faris
    Pages 165-184

  14. Forward and Reverse Genetic Analysis of Chlamydia

    • Mateusz Kędzior, Robert J. Bastidas
    Pages 185-204

  15. A Coinfection Model to Evaluate Chlamydia Inc Protein Interactions

    • Rachel Ende, Isabelle Derré
    Pages 205-218

  16. Rodent Infections for Chlamydia spp.

    • Charles W. Armitage, Alison J. Carey, Kenneth W. Beagley
    Pages 219-236

  17. Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy

    • Steven Liang, James B. Mahony
    Pages 237-244

  18. Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane

    • Macy G. Olson, Lisa M. Jorgenson, Ray E. Widner, Elizabeth A. Rucks
    Pages 245-278

  19. High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, Including Chlamydia trachomatis

    • Amanda Claire Brown, Nicole L. Kushner
    Pages 279-286

  20. Back Matter

    Pages 287-289
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Keywords

About this book

This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and descriptions of genotyping technologies, as well as dual RNA-Seq transcriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.

Editors and Affiliations

  • Department of Microbiology and Immunology, Vet School, Cornell University, Ithaca, USA

    Amanda Claire Brown

Bibliographic Information

  • Book Title: Chlamydia trachomatis

  • Book Subtitle: Methods and Protocols

  • Editors: Amanda Claire Brown

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9694-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9693-3Published: 05 August 2019

  • Softcover ISBN: 978-1-4939-9696-4Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9694-0Published: 05 August 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 289

  • Number of Illustrations: 7 b/w illustrations, 26 illustrations in colour

  • Topics: Medical Microbiology, Infectious Diseases, Pharmaceutical Sciences/Technology, Bacteriology

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