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Methods in Molecular Biology

Computer Optimized Microscopy

Methods and Protocols

Editors: Rebollo, Elena, Bosch, Manel (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9686-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9685-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume explores open-source based image analysis techniques to provide a state-of-the-art collection of workflows covering current bioimage analysis problematics, including colocalization, particle counting, 3D structural analysis, ratio imaging and FRET quantification, particle tracking, high-content screening or machine learning. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and scripts, step-by-step, readily reproducible image analysis protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Computer Optimized Microscopy: Methods and Protocols is a valuable resource for both novice and experienced researchers who are interested in learning more about this developing field. 


Table of contents (21 chapters)

Table of contents (21 chapters)
  • Main Steps in Image Processing and Quantification: The Analysis Workflow

    Pages 3-21

    Pertusa, José F. (et al.)

  • Open Source Tools for Biological Image Analysis

    Pages 23-37

    Guiet, Romain (et al.)

  • Proximity Ligation Assay Image Analysis Protocol: Addressing Receptor-Receptor Interactions

    Pages 41-50

    López-Cano, Marc (et al.)

  • Introduction to ImageJ Macro Language in a Particle Counting Analysis: Automation Matters

    Pages 51-70

    Bosch, Manel

  • Automated Macro Approach to Quantify Synapse Density in 2D Confocal Images from Fixed Immunolabeled Neural Tissue Sections

    Pages 71-97

    Rebollo, Elena (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9686-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9685-8
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Computer Optimized Microscopy
Book Subtitle
Methods and Protocols
Editors
  • Elena Rebollo
  • Manel Bosch
Series Title
Methods in Molecular Biology
Series Volume
2040
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9686-5
DOI
10.1007/978-1-4939-9686-5
Hardcover ISBN
978-1-4939-9685-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 467
Number of Illustrations
8 b/w illustrations, 181 illustrations in colour
Topics