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Methods in Molecular Biology

Protein Self-Assembly

Methods and Protocols

Editors: McManus, Jennifer J. (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-9678-0
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Hardcover $199.99
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About this book

This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field. 


Table of contents (17 chapters)

Table of contents (17 chapters)
  • Measuring Nonspecific Protein–Protein Interactions by Dynamic Light Scattering

    Pages 3-21

    Corbett, Daniel (et al.)

  • Light Scattering to Quantify Protein–Protein Interactions at High Protein Concentrations

    Pages 23-37

    Woldeyes, Mahlet A. (et al.)

  • Quantitative Evaluation of Protein Solubility in Aqueous Solutions by PEG-Induced Liquid–Liquid Phase Separation

    Pages 39-49

    Wang, Ying (et al.)

  • Measuring Protein Solubility

    Pages 51-57

    Asherie, Neer

  • Integral caa3-Cytochrome c Oxidase from Thermus thermophilus: Purification and Crystallization

    Pages 61-79

    Slattery, Orla (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9678-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9677-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Self-Assembly
Book Subtitle
Methods and Protocols
Editors
  • Jennifer J. McManus
Series Title
Methods in Molecular Biology
Series Volume
2039
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9678-0
DOI
10.1007/978-1-4939-9678-0
Hardcover ISBN
978-1-4939-9677-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 266
Number of Illustrations
28 b/w illustrations, 59 illustrations in colour
Topics