Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2039)
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Table of contents (18 protocols)
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Front Matter
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Measuring Protein-Protein Interactions and Protein Phase Diagrams
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Front Matter
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Measuring Protein Self-Association, Aggregation, and Crystallization
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Front Matter
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Computational Approaches to Measure Protein Self-Assembly
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Front Matter
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Keywords
About this book
This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Editors and Affiliations
Bibliographic Information
Book Title: Protein Self-Assembly
Book Subtitle: Methods and Protocols
Editors: Jennifer J. McManus
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9678-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-9677-3Published: 25 July 2019
Softcover ISBN: 978-1-4939-9680-3Published: 14 August 2020
eBook ISBN: 978-1-4939-9678-0Published: 24 July 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 266
Number of Illustrations: 28 b/w illustrations, 59 illustrations in colour
Topics: Protein Science