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Methods in Molecular Biology

Imaging Gene Expression

Methods and Protocols

Editors: Shav-Tal, Yaron (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-9674-2
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Hardcover $199.99
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About this book

This volume explores the latest updates on microscopy approaches and techniques used by scientists studying in the field of gene expression imaging. These updates cover the technical design of the experiments and the expected outcomes. The chapters in this book are divided into two parts: Part One looks at the output of a gene, in particular the RNA molecules that are copied from the gene itself; and Part Two focuses on chromosomes, chromatin, and factors that bind DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Imaging Gene Expression: Methods and Protocols, Second Edition is a valuable resource for any researcher interested in learning more about this evolving and important field. 

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Imaging Single mRNA Molecules in Mammalian Cells Using an Optimized MS2-MCP System

    Pages 3-20

    Vera, Maria (et al.)

  • Live-Cell Imaging of Long Noncoding RNAs Using Molecular Beacons

    Pages 21-33

    Ying, Yachen (et al.)

  • Real-Time Observation of Localization and Expression (ROLEX) System for Live Imaging of the Transcriptional Activity and Nuclear Position of a Specific Endogenous Gene

    Pages 35-45

    Ochiai, Hiroshi

  • Visualization of Single mRNAs in Live Neurons

    Pages 47-61

    Shim, Jae Youn (et al.)

  • Lighting Up Gene Activation in Living Drosophila Embryos

    Pages 63-74

    Fernandez, Carola (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9674-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9673-5
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Imaging Gene Expression
Book Subtitle
Methods and Protocols
Editors
  • Yaron Shav-Tal
Series Title
Methods in Molecular Biology
Series Volume
2038
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9674-2
DOI
10.1007/978-1-4939-9674-2
Hardcover ISBN
978-1-4939-9673-5
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIII, 272
Number of Illustrations
1 b/w illustrations, 46 illustrations in colour
Topics