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Neuromethods

Neuroproteomics

Editors: Li, Ka Wan (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9662-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
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  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9661-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This second edition volume expands on the previous edition with updates on the latest techniques used in the field of proteomics. Tissue and cellular proteins are now being analyzed at near proteome-wide levels and mass spectrometers are achieving higher sensitivity and speed, resolution, and accuracy. The chapters in this book cover various new methods used in proteomic workflows such as laser microdissection for spatial proteomics of post-mortem brain tissue, sample preparation in single vessel with FASP and SP3 protocols, the crosslinking mass spectrometry, TMT-MS3 based proteomic with synchronous precursor selection, mass spectrometric data dependent acquisition (DDA) and data independent acquisition (DIA, SWATH) methods, 2D-DIGE, and MALDI Imaging Mass Spectrometry. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and comprehensive, Neuroproteomics, Second Edition is a valuable guide that help researchers obtain the finest results from current proteomics platforms.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Neuroproteomics: The Methods

    Pages 1-6

    Li, Ka Wan

  • Dissection of Rodent Brain Regions: Guided Free-Hand Slicing and Dissection of Frozen Tissue

    Pages 7-19

    Spijker, Sabine (et al.)

  • Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation: Purification of the Synaptosome, Synaptic Plasma Membrane, Postsynaptic Density, Synaptic Membrane Raft, and Postsynaptic Density Lattice

    Pages 21-42

    Suzuki, Tatsuo (et al.)

  • Laser Microdissection for Spatial Proteomics of Postmortem Brain Tissue

    Pages 43-53

    Hondius, David C. (et al.)

  • High-pH Reversed-Phase Pre-Fractionation for In-Depth Shotgun Proteomics

    Pages 55-65

    Chen, Ning (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9662-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9661-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Neuroproteomics
Editors
  • Ka Wan Li
Series Title
Neuromethods
Series Volume
146
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9662-9
DOI
10.1007/978-1-4939-9662-9
Hardcover ISBN
978-1-4939-9661-2
Series ISSN
0893-2336
Edition Number
2
Number of Pages
XIII, 218
Number of Illustrations
18 b/w illustrations, 37 illustrations in colour
Topics