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Methods in Molecular Biology

Bioconjugation

Methods and Protocols

Editors: Massa, Sam, Devoogdt, Nick (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $169.00
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  • ISBN 978-1-4939-9654-4
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Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9653-7
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About this book

This book explores well-established and emerging conjugation strategies that are relevant for proteins used in the field of precision medicine, focusing on techniques that are suitable for antibodies, antibody-fragments such as Fabs, scFvs, or nanobodies, scaffold proteins such as FN3 or DARPin, peptides, or model proteins. Although centered on the development of bioconjugates rather than their application, most protocols also show the conjugation of the targeting vehicle to a diagnostic or therapeutic entity, with the end-product most often being an antibody-drug conjugate, an optical probe, a nanomedicine, or a radiopharmaceutical. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Bioconjugation: Methods and Protocols is an ideal guide for researchers looking toward precision medicine in order to expand the vital field of drug discovery.

Table of contents (20 chapters)

Table of contents (20 chapters)
  • Site-Specific Conjugation of Thiol-Reactive Cytotoxic Agents to Nonnative Cysteines of Engineered Monoclonal Antibodies

    Pages 1-14

    Dickgiesser, Stephan (et al.)

  • Application of Next-Generation Maleimides (NGMs) to Site-Selective Antibody Conjugation

    Pages 15-24

    Morais, Maurício (et al.)

  • Lysine Bioconjugation on Native Albumin with a Sulfonyl Acrylate Reagent

    Pages 25-37

    Matos, Maria J. (et al.)

  • Engineering Dual Variable Domains for the Generation of Site-Specific Antibody–Drug Conjugates

    Pages 39-52

    Nanna, Alex R. (et al.)

  • Efficient Production of Homogeneous Lysine-Based Antibody Conjugates Using Microbial Transglutaminase

    Pages 53-65

    Spidel, Jared L. (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9654-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9653-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Bioconjugation
Book Subtitle
Methods and Protocols
Editors
  • Sam Massa
  • Nick Devoogdt
Series Title
Methods in Molecular Biology
Series Volume
2033
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9654-4
DOI
10.1007/978-1-4939-9654-4
Hardcover ISBN
978-1-4939-9653-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 317
Number of Illustrations
37 b/w illustrations, 47 illustrations in colour
Topics