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  • © 2019

Immunophenotyping

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2032)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-x
  2. Immunophenotyping by Mass Cytometry

    • Gregory K. Behbehani
    Pages 31-51
  3. Fluorescent Cell Barcoding for Immunophenotyping

    • Valentina Giudice, Giovanna Fantoni, Angélique Biancotto
    Pages 53-68
  4. Guidelines for Gating Flow Cytometry Data for Immunological Assays

    • Janet Staats, Anagha Divekar, J. Philip McCoy Jr., Holden T. Maecker
    Pages 81-104
  5. Strategies and Techniques for NK Cell Phenotyping

    • Chen Ziqing, Andreas Lundqvist, Kristina Witt
    Pages 105-114
  6. Immunophenotyping of Human B Lymphocytes in Blood and in Adipose Tissue

    • Alain Diaz, Maria Romero, Daniela Frasca, Bonnie B. Blomberg
    Pages 115-127
  7. Multiparametric Flow Cytometry Analysis of Naïve, Memory, and Effector T Cells

    • Ankit Saxena, Pradeep K. Dagur, Angélique Biancotto
    Pages 129-140
  8. Immunophenotyping of Human Innate Lymphoid Cells

    • Sara Trabanelli, Alejandra Gomez-Cadena, Camilla Jandus
    Pages 179-192
  9. Enumeration of Plasmacytoid Dendritic Cells in Peripheral Blood and Bone Marrow by Flow-Cytometric Analysis

    • Abdullah Alsuwaidan, Franklin Fuda, Weina Chen, Mingyi Chen
    Pages 193-201
  10. Rare Event Phenotyping and Molecular Characterization: Circulating Tumor Cells

    • Moen Sen, Ling Wang, Liping Yu, Erica L. Carpenter
    Pages 213-226
  11. Quality Control of Immunophenotyping

    • Bruce Greig
    Pages 227-279
  12. Immunophenotyping of Acute Myeloid Leukemia

    • Pallavi Kanwar Galera, Chunjie Jiang, Raul Braylan
    Pages 281-296
  13. Immunophenotyping of Acute Lymphoblastic Leukemia

    • Joseph A. DiGiuseppe, Jolene L. Cardinali
    Pages 297-310
  14. Clinical Flow-Cytometric Testing in Chronic Lymphocytic Leukemia

    • Dalia A. Salem, Maryalice Stetler-Stevenson
    Pages 311-321
  15. Immunophenotyping of Paroxysmal Nocturnal Hemoglobinuria (PNH)

    • Andrea J. Illingworth, Iuri Marinov, D. Robert Sutherland
    Pages 323-354
  16. Back Matter

    Pages 355-356

About this book

This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolvingfield. 



Editors and Affiliations

  • Frederick, USA

    J. Philip McCoy, Jr

Bibliographic Information

  • Book Title: Immunophenotyping

  • Book Subtitle: Methods and Protocols

  • Editors: J. Philip McCoy, Jr

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9650-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9649-0Published: 15 September 2019

  • Softcover ISBN: 978-1-4939-9652-0Published: 15 September 2020

  • eBook ISBN: 978-1-4939-9650-6Published: 14 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 356

  • Number of Illustrations: 15 b/w illustrations, 79 illustrations in colour

  • Topics: Immunology, Cell Biology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access