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Methods in Molecular Biology

Progenitor Cells

Methods and Protocols

Editors: Joglekar, Mugdha V., Hardikar, Anandwardhan A. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9631-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9630-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells. The chapters in this book cover topics such as isolating progenitor cells from sources including adipose tissue, blood, bone marrow, ear, gut, heart, pancreatic islets, and Whirton’s jelly; deriving neural, pancreatic, and urothelial precursors from the embryonic stem cells; and culturing isolated islets in vitro to generate progenitor cells via epithelial-to-mesenchymal transition. This book also delves into digital droplet PCR; flow cytometry and cell sorting; mitochondrial assays; calcium ratiometric signaling; and 2D gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Progenitor Cells: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving and diverse field. 

Table of contents (21 chapters)

Table of contents (21 chapters)
  • Isolation and Molecular Characterization of Progenitor Cells from Human Umbilical Cord

    Pages 1-13

    Goyal, Umesh (et al.)

  • Isolation and Characterization of Extracellular Vesicles from Mesenchymal Stromal Cells

    Pages 15-23

    Kim, Sally Yunsun (et al.)

  • Assessment of β-Cell Replication in Isolated Rat Islets of Langerhans

    Pages 25-35

    Dalgaard, Louise T.

  • Droplet Digital PCR for Measuring Absolute Copies of Gene Transcripts in Human Islet-Derived Progenitor Cells

    Pages 37-48

    Maynard, Cody-Lee (et al.)

  • Isolation and In Vitro Culture of Human Gut Progenitor Cells

    Pages 49-62

    Bruce, Jessica (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9631-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9630-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Progenitor Cells
Book Subtitle
Methods and Protocols
Editors
  • Mugdha V. Joglekar
  • Anandwardhan A. Hardikar
Series Title
Methods in Molecular Biology
Series Volume
2029
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9631-5
DOI
10.1007/978-1-4939-9631-5
Hardcover ISBN
978-1-4939-9630-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 289
Number of Illustrations
11 b/w illustrations, 57 illustrations in colour
Topics