Overview
- Editors:
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Mugdha V. Joglekar
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NHMRC Clinical Trials Centre, Faculty of Medicine and Health, The University of Sydney, Camperdown, Australia
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Anandwardhan A. Hardikar
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NHMRC Clinical Trials Centre, Faculty of Medicine and Health, The University of Sydney, Camperdown, Australia
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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Table of contents (21 protocols)
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- Umesh Goyal, Ankita Sen, Malancha Ta
Pages 1-13
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- Sally Yunsun Kim, Thanh Huyen Phan, Christina Limantoro, Bill Kalionis, Wojciech Chrzanowski
Pages 15-23
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- Cody-Lee Maynard, Wilson K. M. Wong, Anandwardhan A. Hardikar, Mugdha V. Joglekar
Pages 37-48
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- Jessica Bruce, Gerard E. Kaiko, Simon Keely
Pages 49-62
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- Janine C. Quijano, Jacob R. Tremblay, Jeffrey Rawson, Hsun Teresa Ku
Pages 63-80
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- Arpita Poddar, Mugdha V. Joglekar, Anandwardhan A. Hardikar, Ravi Shukla
Pages 81-91
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- Luke Carroll, Auvro R. Mridha, Bernard E. Tuch
Pages 93-102
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- Badwi B. Boumelhem, Stuart T. Fraser, Stephen J. Assinder
Pages 103-115
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- Nitya Shree, Ramesh Bhonde
Pages 117-123
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- Chanukya K. Colonne, Jia Hao Yeo, Campbell V. McKenzie, Stuart T. Fraser
Pages 125-145
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- Ashwini S. Hinge, Marie-Dominique Filippi
Pages 147-160
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- Parul Dixit, Rajesh G. Katare
Pages 161-173
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- Ayeshah A. Rosdah, Lea M. D. Delbridge, Shiang Y. Lim
Pages 175-183
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- Amaresh K. Ranjan, Anil Gulati
Pages 185-195
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- Dario Gerace, Binhai Ren, Rosetta Martiniello-Wilks, Ann M. Simpson
Pages 197-214
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- Kisha N. Sivanathan, Patrick T. Coates
Pages 215-234
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- Sangeetha Vadakke-Madathil, Lalita S. Limaye, Vaijayanti P. Kale, Hina W. Chaudhry
Pages 235-246
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- Lawrence J. Liew, Allen Y. Wang, Rodney J. Dilley
Pages 247-255
About this book
This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells. The chapters in this book cover topics such as isolating progenitor cells from sources including adipose tissue, blood, bone marrow, ear, gut, heart, pancreatic islets, and Whirton’s jelly; deriving neural, pancreatic, and urothelial precursors from the embryonic stem cells; and culturing isolated islets in vitro to generate progenitor cells via epithelial-to-mesenchymal transition. This book also delves into digital droplet PCR; flow cytometry and cell sorting; mitochondrial assays; calcium ratiometric signaling; and 2D gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Progenitor Cells: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving and diverse field.
