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Methods in Molecular Biology

High-Throughput Protein Production and Purification

Methods and Protocols

Editors: Vincentelli, Renaud (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $229.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9624-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $299.99
price for USA in USD
  • ISBN 978-1-4939-9623-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.

Table of contents (24 chapters)

Table of contents (24 chapters)
  • Overview of High-Throughput Cloning Methods for the Post-genomic Era

    Pages 3-32

    Ortega, Claudia (et al.)

  • Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins

    Pages 33-49

    Nettleship, Joanne E. (et al.)

  • Semiautomated Small-Scale Purification Method for High-Throughput Expression Analysis of Recombinant Proteins

    Pages 51-68

    Kraft, Edward (et al.)

  • High-Throughput Protein Production in Yeast

    Pages 69-91

    Fernández, Francisco J. (et al.)

  • A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells

    Pages 93-142

    Rue, Sarah M. (et al.)

Buy this book

eBook $229.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9624-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $299.99
price for USA in USD
  • ISBN 978-1-4939-9623-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
High-Throughput Protein Production and Purification
Book Subtitle
Methods and Protocols
Editors
  • Renaud Vincentelli
Series Title
Methods in Molecular Biology
Series Volume
2025
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9624-7
DOI
10.1007/978-1-4939-9624-7
Hardcover ISBN
978-1-4939-9623-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 537
Number of Illustrations
39 b/w illustrations, 106 illustrations in colour
Topics