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Methods in Molecular Biology

Phytochromes

Methods and Protocols

Editors: Hiltbrunner, Andreas (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9612-4
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Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9611-7
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  • Usually dispatched within 3 to 5 business days.
About this book

This volume aims at providing readers with protocols to enhance their research for the next decade of phytochrome research. The chapters in this book cover topics such as tests for light-dependent interaction of phytochrome binding proteins using yeast two hybrid assays, in vitro pull down, and co-immunoprecipitation from stable transgenic lines; investigations into phosphorylation, SUMOylation, and ubiquitination of phytochromes and other proteins; a look at how in vivo spectroscopy can be used to measure Pfr levels of phytochrome in planta; identifying hormonal action and co-action in light-regulated processes; and ways to use the CRISPR/Cas9 system to generate Physcomitrella mutant lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Phytochromes: Methods and Protocols serves as a guideline to researchers who are new to the field, and a stepping stone for experienced researchers looking to expand their knowledge of this evolving field. 

 


Table of contents (21 chapters)

Table of contents (21 chapters)
  • Investigation of Light-Regulated Protein–Protein Interactions Using Yeast Two-Hybrid Assays

    Pages 1-19

    Sheerin, David J.

  • Rapid Examination of Phytochrome–Phytochrome Interacting Factor (PIF) Interaction by In Vitro Coimmunoprecipitation Assay

    Pages 21-28

    Paik, Inyup (et al.)

  • Characterization of Light-Regulated Protein–Protein Interactions by In Vivo Coimmunoprecipitation (Co-IP) Assays in Plants

    Pages 29-39

    Zhu, Ling (et al.)

  • Detection of Phytochrome Phosphorylation in Plants

    Pages 41-67

    Klement, Eva (et al.)

  • Detection of SUMOylated Phytochromes in Plants

    Pages 69-83

    Orosa, Beatriz (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9612-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9611-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Phytochromes
Book Subtitle
Methods and Protocols
Editors
  • Andreas Hiltbrunner
Series Title
Methods in Molecular Biology
Series Volume
2026
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9612-4
DOI
10.1007/978-1-4939-9612-4
Hardcover ISBN
978-1-4939-9611-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 278
Number of Illustrations
10 b/w illustrations, 30 illustrations in colour
Topics