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Proteus mirabilis

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Melanie M. Pearson

    1. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2021)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Proteus mirabilis Overview

    • Harry L. T. Mobley
    Pages 1-4

  3. Culture Methods for Proteus mirabilis

    • Melanie M. Pearson
    Pages 5-13

  4. Methods for Studying Swarming and Swimming Motility

    • Melanie M. Pearson
    Pages 15-25

  5. Testing the Ability of Compounds to Induce Swarming

    • Chelsie E. Armbruster
    Pages 27-34

  6. Purification of Native Flagellin

    • María José González, Victoria Iribarnegaray, Pablo Zunino, Paola Scavone
    Pages 35-44

  7. Analysis of Proteus mirabilis Social Behaviors on Surfaces

    • Kristin Little, Karine A. Gibbs
    Pages 45-59

  8. Insertional Mutagenesis Protocol for Constructing Single or Sequential Mutations

    • Melanie M. Pearson, Stephanie D. Himpsl, Harry L. T. Mobley
    Pages 61-76

  9. Allelic Exchange Mutagenesis in Proteus mirabilis

    • Kristen E. Howery, Philip N. Rather
    Pages 77-84

  10. Quantification of Urease Activity

    • Shawn Richmond, Alejandra Yep
    Pages 85-96

  11. Siderophore Detection Using Chrome Azurol S and Cross-Feeding Assays

    • Stephanie D. Himpsl, Harry L. T. Mobley
    Pages 97-108

  12. Using Hemagglutination, Surface Shearing, and Acid Treatment to Study Fimbriae in Proteus mirabilis

    • Stephanie D. Himpsl, Melanie M. Pearson, Harry L. T. Mobley
    Pages 109-120

  13. Phase Variation of the mrp Fimbrial Promoter

    • Melanie M. Pearson
    Pages 121-127

  14. Adherence of Proteus mirabilis to Uroepithelial Cells

    • María José González, Victoria Iribarnegaray, Paola Scavone, Pablo Zunino
    Pages 129-137

  15. An In Vitro Bladder Model for Studying Catheter-Associated Urinary Tract Infection and Associated Analysis of Biofilms

    • Jonathan Nzakizwanayo, Harriet Pelling, Scarlet Milo, Brian V. Jones
    Pages 139-158

  16. Independent Transurethral Urinary Tract Inoculation in a Murine Model of Ascending Infection with Proteus mirabilis

    • Sara N. Smith
    Pages 159-172

  17. Cochallenge Inoculation with Proteus mirabilis in a Murine Transurethral Urinary Tract Model of Ascending Infection

    • Sara N. Smith
    Pages 173-186

  18. Indwelling Urinary Catheter Model of Proteus mirabilis Infection

    • Sara N. Smith, Chelsie E. Armbruster
    Pages 187-200

  19. Vaccination to Protect Against Proteus mirabilis Challenge Utilizing the Ascending Model of Urinary Tract Infection

    • Sara N. Smith, Stephanie D. Himpsl, Harry L. T. Mobley
    Pages 201-215

  20. Characterization of Proteus mirabilis Lipopolysaccharide Samples by Infrared Spectroscopy and Serological Methods

    • Katarzyna Durlik, Grzegorz Czerwonka, Paulina Å»arnowiec, WiesÅ‚aw Kaca
    Pages 217-230
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Keywords

About this book

This detailed volume explores essential protocols for the study of Proteus mirabilis which, despite its genetic relatedness to species such as E. coli, often requires specialized handling techniques. This opportunistic bacterial pathogen, most often known as a causative agent of complicated urinary tract infection, is addressed in chapters from global experts in the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Proteus mirabilis: Methods and Protocols serves as an ideal guide for researchers intrigued by the renewed appreciation for the medical impact and environmental adaptability of this organism, coupled with continued fascination for its dynamic behavior.

Editors and Affiliations

  • Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, USA

    Melanie M. Pearson

Bibliographic Information

  • Book Title: Proteus mirabilis

  • Book Subtitle: Methods and Protocols

  • Editors: Melanie M. Pearson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9601-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9600-1Published: 16 July 2019

  • Softcover ISBN: 978-1-4939-9603-2Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9601-8Published: 15 July 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 342

  • Number of Illustrations: 10 b/w illustrations, 46 illustrations in colour

  • Topics: Bacteriology, Medical Microbiology, Pathology

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