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Methods in Molecular Biology

Stem Cell Mobilization

Methods and Protocols

Editors: Klein, Gerd, Wuchter, Patrick (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $149.00
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  • ISBN 978-1-4939-9574-5
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  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9573-8
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  • Usually dispatched within 3 to 5 business days.
About this book

This book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers cell adhesion and migration assays in three-dimensional models, imaging techniques for the determination of blood flow or hypoxic status, innovative biomechanical studies of stem cells, and assays to determine the involvement of proteases or complement factors, as well as other key topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Stem Cell Mobilization: Methods and Protocols is an ideal guide for all laboratories in their endeavors to decipher the mechanisms of stem cell mobilization, ultimately helping stem cell donors and patients around the world.

Table of contents (17 chapters)

Table of contents (17 chapters)
  • Evolution of Peripheral Blood Stem Cell Transplantation

    Pages 1-10

    Ho, Anthony D.

  • In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents

    Pages 11-27

    Tanaka, Motomu

  • Colony Formation: An Assay of Hematopoietic Progenitor Cells

    Pages 29-40

    Kronstein-Wiedemann, Romy (et al.)

  • Mobilization and Collection of Peripheral Blood Stem Cells in Adults: Focus on Timing and Benchmarking

    Pages 41-58

    Kriegsmann, Katharina (et al.)

  • Transmigration Assays for the Determination of Molecular Interactions Between Hematopoietic Stem Cells and Niche Cells

    Pages 59-70

    Henschler, Reinhard (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9574-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9573-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Stem Cell Mobilization
Book Subtitle
Methods and Protocols
Editors
  • Gerd Klein
  • Patrick Wuchter
Series Title
Methods in Molecular Biology
Series Volume
2017
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9574-5
DOI
10.1007/978-1-4939-9574-5
Hardcover ISBN
978-1-4939-9573-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 248
Number of Illustrations
12 b/w illustrations, 54 illustrations in colour
Topics