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Methods in Molecular Biology

Microbial Transposon Mutagenesis

Protocols and Applications

Editors: Ricke, Steven C., Park, Si Hong, Davis, Morgan L. (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-4939-9570-7
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Hardcover $199.99
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  • ISBN 978-1-4939-9569-1
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  • Usually dispatched within 3 to 5 business days.
About this book

This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Preparation of Transposon Library and Tn-Seq Amplicon Library for Salmonella Typhimurium

    Pages 3-15

    Karash, Sardar (et al.)

  • Application of Whole-Genome Sequencing to Transposon Mutants of Salmonella Heidelberg

    Pages 17-27

    Rackerby, Bryna (et al.)

  • Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus

    Pages 29-37

    Endres, Jennifer L. (et al.)

  • Transposon Mutagenesis in Streptococcus Species

    Pages 39-49

    Nilsson, Martin (et al.)

  • Implementation of Transposon Mutagenesis in Bifidobacterium

    Pages 51-62

    Ruiz, Lorena (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9570-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9569-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Microbial Transposon Mutagenesis
Book Subtitle
Protocols and Applications
Editors
  • Steven C. Ricke
  • Si Hong Park
  • Morgan L. Davis
Series Title
Methods in Molecular Biology
Series Volume
2016
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9570-7
DOI
10.1007/978-1-4939-9570-7
Hardcover ISBN
978-1-4939-9569-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 183
Number of Illustrations
12 b/w illustrations, 34 illustrations in colour
Topics