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Methods in Molecular Biology

Comparative Embryo Culture

Methods and Protocols

Editors: Herrick, Jason R. (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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About this book

This detailed volume contains embryo culture techniques that should inspire embryologists to consider comparative studies. The species included in this volume represent a broad range of taxa, whose embryos have vastly different culture requirements and developmental characteristics.  Among the species described in this volume, significant differences exist in the rates of cell division, the timing of the maternal to embryonic transition, the relative lipid content of the cytoplasm, the sensitivity of the embryo to specific environmental ions, the preferred nutrients and associated metabolic pathways used by the embryo, the timing and mechanisms of early lineage specification, the presence or absence of embryonic diapause, and the time from fertilization to implantation. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Comparative Embryo Culture: Methods and Protocols serves as an ideal aid for scientists seeking another species whose embryos have evolved a unique mechanism that could provide a valuable, comparative context for experimentation.

Table of contents (25 chapters)

Table of contents (25 chapters)
  • Historical Note: Lessons Learned from Developing the First Semi-Defined Medium for Culture of One-Cell Rabbit Embryos to Expanding Blastocysts

    Pages 1-12

    Kane, Michael T.

  • Culture of the Mouse Preimplantation Embryo

    Pages 13-32

    Gardner, David K. (et al.)

  • In Vitro Culture of Rat Preimplantation Embryos

    Pages 33-43

    Agca, Yuksel

  • Enabling Hamster Embryo Culture System: Development of Preimplantation Embryos

    Pages 45-61

    Seshagiri, Polani B. (et al.)

  • Culture of Preimplantation Rabbit Embryos

    Pages 63-91

    Kane, Michael T.

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-4939-9566-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9565-3
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
Softcover $149.99
price for USA in USD
  • ISBN 978-1-4939-9568-4
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually ready to be dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Comparative Embryo Culture
Book Subtitle
Methods and Protocols
Editors
  • Jason R. Herrick
Series Title
Methods in Molecular Biology
Series Volume
2006
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9566-0
DOI
10.1007/978-1-4939-9566-0
Hardcover ISBN
978-1-4939-9565-3
Softcover ISBN
978-1-4939-9568-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 397
Number of Illustrations
23 b/w illustrations, 42 illustrations in colour
Topics

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