- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Buy this book
- About this book
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This detailed book presents methods that enable the determination of parameters relevant to phloem research in the most efficient ways, putting plant scientists with access to adequate instrumentation in the position to answer any phloem-related question. The collection explores techniques that have been used for decades, such as tracing phloem transport with carbon isotopes, as well as recent developments, such as esculin-based assays of phloem transport and super-resolution microscopy of phloem proteins. As such, the book presents the state-of-the-art in phloem research and, at the same time, a starting point for the development of new methods that will fill the remaining gaps in the phloem researcher’s toolbox in the future. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Phloem: Methods and Protocols is an ideal guide for all researchers exploring this challenging area of plant biology.
- Table of contents (36 chapters)
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Optimal Preparation of Tissue Sections for Light-Microscopic Analysis of Phloem Anatomy
Pages 3-16
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Transmission Electron Microscopy of the Phloem with Minimal Artifacts
Pages 17-27
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Scanning Electron Microscopy of the Phloem
Pages 29-35
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Noninvasive Investigation of Phloem Structure by 3D Synchrotron X-Ray Microtomography
Pages 37-54
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Quantification of Leaf Phloem Anatomical Features with Microscopy
Pages 55-72
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Table of contents (36 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Phloem
- Book Subtitle
- Methods and Protocols
- Editors
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- Johannes Liesche
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2014
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9562-2
- DOI
- 10.1007/978-1-4939-9562-2
- Hardcover ISBN
- 978-1-4939-9561-5
- Softcover ISBN
- 978-1-4939-9564-6
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIV, 473
- Number of Illustrations
- 20 b/w illustrations, 87 illustrations in colour
- Topics
