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Methods in Molecular Biology

Enzyme-Mediated Ligation Methods

Editors: Nuijens, Timo, Schmidt, Marcel (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $119.00
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  • ISBN 978-1-4939-9546-2
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Hardcover $159.99
price for USA in USD
  • ISBN 978-1-4939-9545-5
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  • Usually dispatched within 3 to 5 business days.
About this book

This volume discusses different enzyme-catalyzed ligation methodologies for a variety of different chemical transformations. This book wants readers to view enzymes as a powerful tool in both academic and industrial research. Chapters in this book cover topics such as sortase A-mediated generation of site-specifically conjugated antibody-drug conjugates; omniligase-catalyzed inter- and intramolecular ligation; ligation catalyzed by microbial transglutaminase; peptide cyclization mediated by cyanobactin macrocyclases, butelase 1 and sortase A; using BioID as a tool for protein proximity labeling in living cells; and inducible, selective labeling of proteins via enzymatic oxidation of tyrosine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Enzyme-Mediated Ligation Methods is a valuable resource for students and scientists from different disciplines who are interested in using enzymatic strategies to answer their research questions. 


Table of contents (18 chapters)

Table of contents (18 chapters)
  • Sortase A Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody–Drug Conjugates

    Pages 1-13

    Gébleux, Rémy (et al.)

  • Site-Specific C-Terminal Labeling of Recombinant Proteins with Proximity-Based Sortase-Mediated Ligation (PBSL)

    Pages 15-28

    Wang, Hejia Henry (et al.)

  • Cyclizing Disulfide-Rich Peptides Using Sortase A

    Pages 29-41

    Agwa, Akello J. (et al.)

  • Chemoenzymatic Synthesis of Linear- and Head-to-Tail Cyclic Peptides Using Omniligase-1

    Pages 43-61

    Schmidt, Marcel (et al.)

  • Site-Specific Labeling of Proteins Using the Formylglycine-Generating Enzyme (FGE)

    Pages 63-81

    Rupniewski, Igor (et al.)

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9546-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-4939-9545-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Enzyme-Mediated Ligation Methods
Editors
  • Timo Nuijens
  • Marcel Schmidt
Series Title
Methods in Molecular Biology
Series Volume
2012
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9546-2
DOI
10.1007/978-1-4939-9546-2
Hardcover ISBN
978-1-4939-9545-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 371
Number of Illustrations
35 b/w illustrations, 59 illustrations in colour
Topics