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Methods in Molecular Biology

Proximity Labeling

Methods and Protocols

Editors: Sunbul, Murat, Jäschke, Andres (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $169.00
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  • ISBN 978-1-4939-9537-0
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Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9536-3
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  • Usually dispatched within 3 to 5 business days.
About this book

This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.

Table of contents (16 chapters)

Table of contents (16 chapters)
  • The EMARS Reaction for Proximity Labeling

    Pages 1-12

    Honke, Koichi (et al.)

  • Proteomic Proximity Labeling to Reveal Interactions Between Biomolecules

    Pages 13-28

    Rees, Johanna Susan

  • Establishing Cell Culture-Based Experimental Setups for Proximity Labeling Using Ascorbate Peroxidase (APEX)

    Pages 29-39

    Mick, David U.

  • APEX Peroxidase-Catalyzed Proximity Labeling and Multiplexed Quantitative Proteomics

    Pages 41-55

    Kalocsay, Marian

  • Identification of Lipid Droplet Proteomes by Proximity Labeling Proteomics Using APEX2

    Pages 57-72

    Bersuker, Kirill (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9537-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9536-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Proximity Labeling
Book Subtitle
Methods and Protocols
Editors
  • Murat Sunbul
  • Andres Jäschke
Series Title
Methods in Molecular Biology
Series Volume
2008
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9537-0
DOI
10.1007/978-1-4939-9537-0
Hardcover ISBN
978-1-4939-9536-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 231
Number of Illustrations
28 b/w illustrations, 46 illustrations in colour
Topics