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Methods in Molecular Biology

Protein Lipidation

Methods and Protocols

Editors: Linder, Maurine (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-4939-9532-5
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Hardcover $219.99
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About this book

This volume explores techniques used to detect lipids attached to proteins, to analyze the function of lipid modifications, and to characterize the enzymes that add and remove lipids from proteins. The book is organized into seven parts: Part One describes chemically-based strategies to identify substrates for protein lipidation that can be applied to individual proteins or globally using proteomics. Part Two focuses on the enzymes that remove fatty acids from proteins and provides methods to monitor protein biogenesis and palmitate turnover. Part Three addresses biochemical and cellular characterization of DHHC S-acyltransferases, a family of enzymes with 23 members encoded by the human genome. Part Four presents the SwissPalm 2 database and tips on how to use it effectively. Part Five focuses on fatty acylation that occurs in the lumen of the secretory pathway. Parts Six and Seven conclude the book with methods to produce and assay lipid-modified and integral membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Protein Lipidation: Methods and Protocols is a valuable resource for experts in the field and for investigators who encounter protein lipidation through their research on a particular cellular process or favorite protein.


Table of contents (24 chapters)

Table of contents (24 chapters)
  • Determination of Protein S-Acylation State by Enhanced Acyl-Switch Methods

    Pages 3-11

    Hurst, Charlotte H. (et al.)

  • Detection of Heterogeneous Protein S-Acylation in Cells

    Pages 13-33

    Greaves, Jennifer (et al.)

  • Optimization of Metabolic Labeling with Alkyne-Containing Isoprenoid Probes

    Pages 35-43

    Ahmadi, Mina (et al.)

  • Chemical Proteomic Analysis of S-Fatty Acylated Proteins and Their Modification Sites

    Pages 45-57

    Thinon, Emmanuelle (et al.)

  • Direct Analysis of Protein S-Acylation by Mass Spectrometry

    Pages 59-70

    Ji, Yuhuan (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9532-5
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9531-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Lipidation
Book Subtitle
Methods and Protocols
Editors
  • Maurine Linder
Series Title
Methods in Molecular Biology
Series Volume
2009
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9532-5
DOI
10.1007/978-1-4939-9532-5
Hardcover ISBN
978-1-4939-9531-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 327
Number of Illustrations
19 b/w illustrations, 44 illustrations in colour
Topics