Vascular Effects of Hydrogen Sulfide

1. Front Matter

   Pages i-x

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2. Synthesis, Metabolism, and Signaling Mechanisms of Hydrogen Sulfide: An Overview

   • Jerzy Bełtowski

   Pages 1-8

3. Lanthionine and Other Relevant Sulfur Amino Acid Metabolites: Detection of Prospective Uremic Toxins in Serum by Multiple Reaction Monitoring Tandem Mass Spectrometry

   • Alessandra F. Perna, Francesca Pane, Nunzio Sepe, Carolina Fontanarosa, Gabriella Pinto, Miriam Zacchia et al.

   Pages 9-17

4. Analysis of Vascular Hydrogen Sulfide Biosynthesis

   • Thomas J. Lechuga, Dong-bao Chen

   Pages 19-36

5. Measurement of Protein Persulfidation: Improved Tag-Switch Method

   • Emilia Kouroussis, Bikash Adhikari, Jasmina Zivanovic, Milos R. Filipovic

   Pages 37-50

6. ProPerDP: A Protein Persulfide Detection Protocol

   • Éva Dóka, Elias S. J. Arnér, Edward E. Schmidt, Péter Nagy

   Pages 51-77

7. Vascular Effects of H2S-Donors: Fluorimetric Detection of H2S Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells

   • Alma Martelli, Valentina Citi, Vincenzo Calderone

   Pages 79-87

8. In Vitro Measurement of H2S-Mediated Vasoactive Responses

   • Sona Cacanyiova, Andrea Berenyiova

   Pages 89-107

9. In Vivo Measurement of H2S, Polysulfides, and “SSNO− Mix”-Mediated Vasoactive Responses and Evaluation of Ten Hemodynamic Parameters from Rat Arterial Pulse Waveform

   • Frantisek Kristek, Marian Grman, Karol Ondrias

   Pages 109-124

10. Simultaneous Measurements of Tension and Free H2S in Mesenteric Arteries

    • Elise Røge Nielsen, Anna K. Winther, Ulf Simonsen

    Pages 125-136

11. The Relaxant Mechanisms of Hydrogen Sulfide in Corpus Cavernosum

    • Fatma Aydinoglu, Nuran Ogulener

    Pages 137-150

12. Pharmacological Tools for the Study of H2S Contribution to Angiogenesis

    • Lucia Morbidelli, Martina Monti, Erika Terzuoli

    Pages 151-166

13. Central Administration of H2S Donors for Studying Cardiovascular Effects of H2S in Rats

    • Marcin Ufnal, Artur Nowinski

    Pages 167-172

14. Colonic Delivery of H2S Donors for Studying Cardiovascular Effects of H2S in Rats

    • Marcin Ufnal, Tomasz Hutsch

    Pages 173-177

15. Measurements for Sulfide-Mediated Inhibition of Myeloperoxidase Activity

    • Dorottya Garai, Zoltán Pálinkás, József Balla, Anthony J. Kettle, Péter Nagy

    Pages 179-203

16. Vascular Myography to Examine Functional Responses of Isolated Blood Vessels

    • Joanne Hart

    Pages 205-217

17. Back Matter

    Pages 219-221

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This book explores techniques for exploring hydrogen sulfide (H₂S) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular H₂S generation/signaling may be involved in the pathogenesis of systemic and pulmonary arterial hypertension, ischemic heart disease, ischemic stroke, preeclampsia, and erectile dysfunction, and H₂S also serves as an attractive target for pharmacotherapy of cardiovascular diseases, as well as possible effects on cancer, wound healing, and diabetic retinopathy, among other pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Vascular Effects of Hydrogen Sulfide: Methods and Protocols is an ideal aid for scientists working to extend our knowledge in this valuable and wide-ranging field of study.

• Endogenous mediator
• Vascular biology
• H2S generation
• Cardiovascular pharmacotherapy
• Vascular pathogenesis

• Department of Pathophysiology, Medical University, Lublin, Poland

  Jerzy Bełtowski

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