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Vascular Effects of Hydrogen Sulfide

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Jerzy Bełtowski

    1. Department of Pathophysiology, Medical University, Lublin, Poland

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2007)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Synthesis, Metabolism, and Signaling Mechanisms of Hydrogen Sulfide: An Overview

    • Jerzy Bełtowski
    Pages 1-8

  3. Lanthionine and Other Relevant Sulfur Amino Acid Metabolites: Detection of Prospective Uremic Toxins in Serum by Multiple Reaction Monitoring Tandem Mass Spectrometry

    • Alessandra F. Perna, Francesca Pane, Nunzio Sepe, Carolina Fontanarosa, Gabriella Pinto, Miriam Zacchia et al.
    Pages 9-17

  4. Analysis of Vascular Hydrogen Sulfide Biosynthesis

    • Thomas J. Lechuga, Dong-bao Chen
    Pages 19-36

  5. Measurement of Protein Persulfidation: Improved Tag-Switch Method

    • Emilia Kouroussis, Bikash Adhikari, Jasmina Zivanovic, Milos R. Filipovic
    Pages 37-50

  6. ProPerDP: A Protein Persulfide Detection Protocol

    • Éva Dóka, Elias S. J. Arnér, Edward E. Schmidt, Péter Nagy
    Pages 51-77

  7. Vascular Effects of H2S-Donors: Fluorimetric Detection of H2S Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells

    • Alma Martelli, Valentina Citi, Vincenzo Calderone
    Pages 79-87

  8. In Vitro Measurement of H2S-Mediated Vasoactive Responses

    • Sona Cacanyiova, Andrea Berenyiova
    Pages 89-107

  9. In Vivo Measurement of H2S, Polysulfides, and “SSNO− Mix”-Mediated Vasoactive Responses and Evaluation of Ten Hemodynamic Parameters from Rat Arterial Pulse Waveform

    • Frantisek Kristek, Marian Grman, Karol Ondrias
    Pages 109-124

  10. Simultaneous Measurements of Tension and Free H2S in Mesenteric Arteries

    • Elise Røge Nielsen, Anna K. Winther, Ulf Simonsen
    Pages 125-136

  11. The Relaxant Mechanisms of Hydrogen Sulfide in Corpus Cavernosum

    • Fatma Aydinoglu, Nuran Ogulener
    Pages 137-150

  12. Pharmacological Tools for the Study of H2S Contribution to Angiogenesis

    • Lucia Morbidelli, Martina Monti, Erika Terzuoli
    Pages 151-166

  13. Central Administration of H2S Donors for Studying Cardiovascular Effects of H2S in Rats

    • Marcin Ufnal, Artur Nowinski
    Pages 167-172

  14. Colonic Delivery of H2S Donors for Studying Cardiovascular Effects of H2S in Rats

    • Marcin Ufnal, Tomasz Hutsch
    Pages 173-177

  15. Measurements for Sulfide-Mediated Inhibition of Myeloperoxidase Activity

    • Dorottya Garai, Zoltán Pálinkás, József Balla, Anthony J. Kettle, Péter Nagy
    Pages 179-203

  16. Vascular Myography to Examine Functional Responses of Isolated Blood Vessels

    • Joanne Hart
    Pages 205-217

  17. Back Matter

    Pages 219-221
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Keywords

About this book

This book explores techniques for exploring hydrogen sulfide (H2S) and its effects on the vascular system through numerous experimental animal models and vascular preparations. Alterations of vascular H2S generation/signaling may be involved in the pathogenesis of systemic and pulmonary arterial hypertension, ischemic heart disease, ischemic stroke, preeclampsia, and erectile dysfunction, and H2S also serves as an attractive target for pharmacotherapy of cardiovascular diseases, as well as possible effects on cancer, wound healing, and diabetic retinopathy, among other pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Vascular Effects of Hydrogen Sulfide: Methods and Protocols is an ideal aid for scientists working to extend our knowledge in this valuable and wide-ranging field of study.

Editors and Affiliations

  • Department of Pathophysiology, Medical University, Lublin, Poland

    Jerzy Bełtowski

Bibliographic Information

  • Book Title: Vascular Effects of Hydrogen Sulfide

  • Book Subtitle: Methods and Protocols

  • Editors: Jerzy Bełtowski

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9528-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9527-1Published: 31 May 2019

  • Softcover ISBN: 978-1-4939-9530-1Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9528-8Published: 30 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 221

  • Number of Illustrations: 23 b/w illustrations, 47 illustrations in colour

  • Topics: Molecular Medicine, Pharmaceutical Sciences/Technology, Cardiovascular Biology

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