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Methods in Molecular Biology

Chimera Research

Methods and Protocols

Editors: Hyun, Insoo, De Los Angeles, Alejandro (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9524-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9523-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume addresses challenging new questions surrounding stem cell-based chimera research. This book is organized into three parts: Part One provides readers with a summary of different human donor cell types. The chapters in this section discuss ways to evaluate new types of pluripotent stem cells; the derivation of naïve and primed pluripotent stem cells from mouse preimplantation embryos; and the ethical and regulatory complexities of informed consent for the procurement of somatic cells. Part Two discusses methods for generating chimeras. The chapters here look at chick models and human-chick organizer grafts; generating human-pig interspecies chimeras; and techniques for transplanting mouse neural stem cells into a mouse disease model for stroke. Part Three concludes the book with a look at ongoing ethical controversies and new scientific directions. Chapters in this part cover the ethics of crossing the xenobarrier; animal welfare; experimentation with spermatogonial stem cells; and cautious approaches to human-monkey chimera studies to further understand complex human brain disorders. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chimera Research: Methods and Protocols is a valuable resource for scientists interesting in using chimeras as a research tool while also taking into consideration their complex ethical scopes. 



Table of contents (15 chapters)

Table of contents (15 chapters)
  • Frontiers of Pluripotency

    Pages 3-27

    De Los Angeles, Alejandro

  • Highly Efficient Derivation of Pluripotent Stem Cells from Mouse Preimplantation and Postimplantation Embryos in Serum-Free Conditions

    Pages 29-36

    De Los Angeles, Alejandro (et al.)

  • Derivation of Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells

    Pages 37-41

    Sugimura, Ryohichi

  • Cancer Stem Cells: Concepts, Challenges, and Opportunities for Cancer Therapy

    Pages 43-66

    Lee, May Yin (et al.)

  • Informed Consent Issues for Cell Donors

    Pages 67-74

    Hyun, Insoo

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9524-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-4939-9523-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Chimera Research
Book Subtitle
Methods and Protocols
Editors
  • Insoo Hyun
  • Alejandro De Los Angeles
Series Title
Methods in Molecular Biology
Series Volume
2005
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9524-0
DOI
10.1007/978-1-4939-9524-0
Hardcover ISBN
978-1-4939-9523-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 234
Number of Illustrations
5 b/w illustrations, 23 illustrations in colour
Topics