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  • © 2019

SMC Complexes

Methods and Protocols

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2004)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
  2. Depletion Systems to Assess SMC Function

    1. Front Matter

      Pages 1-1
    2. Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System

      • Clémentine Brocas, Cécile Ducrot, Karine Dubrana
      Pages 17-24
    3. Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts

      • Himaja Gaddipati, Marina V. Pryzhkova, Philip W. Jordan
      Pages 35-46
  3. Genetic Manipulation of SMC Function

    1. Front Matter

      Pages 47-47
    2. High-Throughput Allelic Replacement Screening in Bacillus subtilis

      • Marie-Laure Diebold-Durand, Frank Bürmann, Stephan Gruber
      Pages 49-61
    3. Multicomponent Yeast Two-Hybrid System: Applications to Study Protein–Protein Interactions in SMC Complexes

      • Jan Josef Paleček, Lucie Vondrová, Kateřina Zábrady, Jakub Otočka
      Pages 79-90
    4. Knocking in Multifunctional Gene Tags into SMC Complex Subunits Using Gene Editing

      • Paul Kalitsis, Tao Zhang, Ji Hun Kim, Christian F. Nielsen, Kathryn M. Marshall, Damien F. Hudson
      Pages 91-102
  4. Chromosomal Assays of SMC Activity

    1. Front Matter

      Pages 103-103
    2. Analysis of the Chromosomal Localization of Yeast SMC Complexes by Chromatin Immunoprecipitation

      • Vasso Makrantoni, Daniel Robertson, Adele L. Marston
      Pages 119-138Open Access
    3. Preparation of Cell Cycle-Synchronized Saccharomyces cerevisiae Cells for Hi-C

      • Stephanie A. Schalbetter, Jonathan Baxter
      Pages 155-165
  5. Biochemical Assays of SMC Activity

    1. Front Matter

      Pages 167-167
    2. Dissecting DNA Compaction by the Bacterial Condensin MukB

      • Rupesh Kumar, Soon Bahng, Kenneth J. Marians
      Pages 169-180
    3. A Protocol for Assaying the ATPase Activity of Recombinant Cohesin Holocomplexes

      • Menelaos Voulgaris, Thomas G. Gligoris
      Pages 197-208

About this book

This book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic Structural Maintenance of Chromosome (SMC) proteins. The protocols explore numerous subjects such as depletion systems to assess SMC function, genetic manipulation of SMC proteins, chromosomal, biochemical, and microscopy-based assays of SMC activity, as well as theoretical modeling of SMC activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, SMC Complexes: Methods and Protocols will greatly aid researchers looking into the mechanism of action of SMC complexes on DNA.


Chapters 10 and 19 areavailable Open Access under a CC BY 4.0 license via link.springer.com.

Editors and Affiliations

  • National Centre for Biological Sciences, Tata Institute of Fundamental Research (TIFR), Bangalore, India

    Anjana Badrinarayanan

Bibliographic Information

  • Book Title: SMC Complexes

  • Book Subtitle: Methods and Protocols

  • Editors: Anjana Badrinarayanan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9520-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9519-6Published: 31 May 2019

  • Softcover ISBN: 978-1-4939-9522-6Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9520-2Published: 30 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 336

  • Number of Illustrations: 36 b/w illustrations, 55 illustrations in colour

  • Topics: Genetics and Genomics, Protein Science

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access