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Methods in Molecular Biology

SMC Complexes

Methods and Protocols

Editors: Badrinarayanan, Anjana (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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  • ISBN 978-1-4939-9520-2
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Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9519-6
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About this book

This book brings together recent methods and theoretical approaches developed to dissect the activity and function of bacterial and eukaryotic Structural Maintenance of Chromosome (SMC) proteins. The protocols explore numerous subjects such as depletion systems to assess SMC function, genetic manipulation of SMC proteins, chromosomal, biochemical, and microscopy-based assays of SMC activity, as well as theoretical modeling of SMC activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, SMC Complexes: Methods and Protocols will greatly aid researchers looking into the mechanism of action of SMC complexes on DNA.
Chapters 10 and 19 are available Open Access under a CC BY 4.0 license via link.springer.com.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Using Cell Cycle-Restricted Alleles to Study the Chromatin Dynamics and Functions of the Structural Maintenance of Chromosomes (SMC) Complexes In Vivo

    Pages 3-16

    Menolfi, Demis (et al.)

  • Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System

    Pages 17-24

    Brocas, Clémentine (et al.)

  • Efficient Depletion of Fission Yeast Condensin by Combined Transcriptional Repression and Auxin-Induced Degradation

    Pages 25-33

    Kakui, Yasutaka (et al.)

  • Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts

    Pages 35-46

    Gaddipati, Himaja (et al.)

  • High-Throughput Allelic Replacement Screening in Bacillus subtilis

    Pages 49-61

    Diebold-Durand, Marie-Laure (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9520-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9519-6
  • with online files
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
SMC Complexes
Book Subtitle
Methods and Protocols
Editors
  • Anjana Badrinarayanan
Series Title
Methods in Molecular Biology
Series Volume
2004
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9520-2
DOI
10.1007/978-1-4939-9520-2
Hardcover ISBN
978-1-4939-9519-6
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 336
Number of Illustrations
36 b/w illustrations, 55 illustrations in colour
Topics