- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
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- About this book
-
This updated collection addresses the intense efforts that have been underway to characterize the cellular-molecular-biochemical elements of not only the hematopoietic stem cell niche but the niches for other stem cells. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the first edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Niche: Methods and Protocols, Second Edition serves as an ideal guide for both experts and novices in the stem cell field.
- Table of contents (15 chapters)
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In Vitro Maintenance of Multipotent Neural Crest Stem Cells as Crestospheres
Pages 1-11
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Analysis of Hematopoietic Niche in the Mouse Embryo
Pages 13-27
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Functional Assays of Stem Cell Properties Derived from Different Niches
Pages 29-38
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Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche
Pages 39-50
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Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment
Pages 51-59
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Table of contents (15 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Stem Cell Niche
- Book Subtitle
- Methods and Protocols
- Editors
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- Kursad Turksen
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2002
- Copyright
- 2019
- Publisher
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-4939-9508-0
- DOI
- 10.1007/978-1-4939-9508-0
- Hardcover ISBN
- 978-1-4939-9507-3
- Softcover ISBN
- 978-1-4939-9510-3
- Series ISSN
- 1064-3745
- Edition Number
- 2
- Number of Pages
- XII, 199
- Number of Illustrations
- 6 b/w illustrations, 29 illustrations in colour
- Topics
