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Methods in Molecular Biology

The ESCRT Complexes

Methods and Protocols

Editors: Culetto, Emmanuel, Legouis, Renaud (Eds.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9492-2
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Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9491-5
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  • Usually dispatched within 3 to 5 business days.
About this book

This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Analysis of the Archaeal ESCRT Apparatus

    Pages 1-11

    Samson, Rachel Y. (et al.)

  • Genetic and Cell Biology Methods to Study ESCRTs in Drosophila melanogaster

    Pages 13-29

    Gualtieri, Marco (et al.)

  • Functional Analysis of ESCRT-Positive Extracellular Vesicles in the Drosophila Wing Imaginal Disc

    Pages 31-47

    Matusek, Tamás (et al.)

  • Subcellular Localization of ESCRT-II in the Nematode C. elegans by Correlative Light Electron Microscopy

    Pages 49-61

    Largeau, Céline (et al.)

  • Proximity Ligation Assay (PLA) to Determine the Endosomal Localization of ESCRT Subunit in Virus-Infected Cells

    Pages 63-72

    Kumar, Binod (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-4939-9492-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9491-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
The ESCRT Complexes
Book Subtitle
Methods and Protocols
Editors
  • Emmanuel Culetto
  • Renaud Legouis
Series Title
Methods in Molecular Biology
Series Volume
1998
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9492-2
DOI
10.1007/978-1-4939-9492-2
Hardcover ISBN
978-1-4939-9491-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 322
Number of Illustrations
29 b/w illustrations, 43 illustrations in colour
Topics