Methods in Molecular Biology

Cell-Based Assays Using iPSCs for Drug Development and Testing

Editors: Mandenius, Carl-Fredrik, Ross, James A (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-4939-9477-9
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About this book

The volume presents a variety of protocols that will be useful to researchers working in the field of disease modeling, drug discovery, and the cell biology of various tissues. Chapters in this book cover topics such as requirements and preconditions for using human induced pluripotent cell lines in assay development; the culture of iPSCs and iPSC-derived cardiomyocytes by bioreactor technologies; distinctions between hepatocytes, vascular endothelial cells, cardiomyocytes, renal podocytes, and neurons; and high content assays of mitochondrial function and autophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Based Assay using iPSCs for Drug Development and Testing: Methods and Protocols is a valuable resource for researchers working in this important and developing field of study.

Table of contents (23 chapters)

Table of contents (23 chapters)
  • Cell-Based Assays Using Differentiated Human Induced Pluripotent Cells

    Pages 1-14

    Cader, Zameel (et al.)

  • Monolayer Generation of Vascular Endothelial Cells from Human Pluripotent Stem Cells

    Pages 17-29

    Christensen, Klaus (et al.)

  • Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling

    Pages 31-39

    Janssen, Katharina (et al.)

  • Human Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Culture and Hepatic Differentiation

    Pages 41-53

    Palakkan, Anwar A. (et al.)

  • Production of Cardiomyocytes from Human Pluripotent Stem Cells by Bioreactor Technologies

    Pages 55-70

    Halloin, Caroline (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-9477-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
Softcover $149.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Cell-Based Assays Using iPSCs for Drug Development and Testing
Editors
  • Carl-Fredrik Mandenius
  • James A Ross
Series Title
Methods in Molecular Biology
Series Volume
1994
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9477-9
DOI
10.1007/978-1-4939-9477-9
Hardcover ISBN
978-1-4939-9476-2
Softcover ISBN
978-1-4939-9479-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 266
Number of Illustrations
14 b/w illustrations, 55 illustrations in colour
Topics