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Plant Cell Morphogenesis

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Fatima Cvrčková

    1. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

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    You can also search for this editor in PubMed   Google Scholar

  2. Viktor Žárský

    1. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1992)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Essential Methods of Plant Sample Preparation for Light Microscopy

    • Aleš Soukup, Edita Tylová
    Pages 1-26

  3. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy

    • Aleš Soukup
    Pages 27-42

  4. Chemical Fixation, Immunofluorescence, and Immunogold Labeling of Electron Microscopical Sections

    • Ilse Foissner, Margit Hoeftberger
    Pages 43-62

  5. Essential Methods of Plant Sample Preparation for High-Resolution Scanning Electron Microscopy at Room Temperature

    • Jana Nebesářová
    Pages 63-76

  6. Fluorescence Lifetime Imaging of Plant Cell Walls

    • Christine Terryn, Gabriel Paës
    Pages 77-82

  7. Raman Spectroscopy in Nonwoody Plants

    • Dorota Borowska-Wykręt, Mateusz Dulski
    Pages 83-107

  8. Image Analysis: Basic Procedures for Description of Plant Structures

    • Jana Albrechtová, Zuzana Kubínová, Aleš Soukup, Jiří Janáček
    Pages 109-119

  9. From Data to Illustrations: Common (Free) Tools for Proper Image Data Handling and Processing

    • Fatima Cvrčková
    Pages 121-133

  10. Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics

    • Amparo Rosero, Denisa Oulehlová, Viktor Žárský, Fatima Cvrčková
    Pages 135-149

  11. Quantitative and Comparative Analysis of Global Patterns of (Microtubule) Cytoskeleton Organization with CytoskeletonAnalyzer2D

    • Birgit Möller, Luise Zergiebel, Katharina Bürstenbinder
    Pages 151-171

  12. Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions

    • Adriana Jelínková, Kateřina Malínská, Jan Petrášek
    Pages 173-187

  13. Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells

    • Lise C. Noack, Přemysl Pejchar, Juraj Sekereš, Yvon Jaillais, Martin Potocký
    Pages 189-199

  14. The Photoconvertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics

    • Alexandra Lešková, Zuzana Kusá, Mária Labajová, Miroslav Krausko, Ján Jásik
    Pages 201-214

  15. Cellular Force Microscopy to Measure Mechanical Forces in Plant Cells

    • Mateusz Majda, Aleksandra Sapala, Anne-Lise Routier-Kierzkowska, Richard S. Smith
    Pages 215-230

  16. Optical Trapping in Plant Cells

    • Tijs Ketelaar, Norbert de Ruijter, Stefan Niehren
    Pages 231-238

  17. Sequential Replicas: Method for In Vivo Imaging of Plant Organ Surfaces that Undergo Deformation

    • Dorota Kwiatkowska, Sandra Natonik-Białoń, Agata Burian
    Pages 239-255

  18. Time-Lapse Imaging of Developing Shoot Meristems Using A Confocal Laser Scanning Microscope

    • Olivier Hamant, Pradeep Das, Agata Burian
    Pages 257-268

  19. Quantifying Plant Growth and Cell Proliferation with MorphoGraphX

    • Soeren Strauss, Aleksandra Sapala, Daniel Kierzkowski, Richard S. Smith
    Pages 269-290

  20. Kinematic Characterization of Root Growth by Means of Stripflow

    • Tobias I. Baskin, Ellen Zelinsky
    Pages 291-305
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Keywords

About this book

This book collects techniques to continue exploring post-genomic land plant biology though the wisdom and skills accumulated from work on the founding molecular biology models that can now guide research into other species, including crop plants. Beginning with the visualization of plant cell structures, the volume moves on to cover digital image analysis protocols, qualitative and quantitative detection of the organization and dynamics of individual intracellular structures, the manipulation of intracellular structures, as well as techniques for studying model cell types. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and fully updated, Plant Cell Morphogenesis: Methods and Protocols, Second Edition serves as an ideal source of inspiration for further research into the morphogenesis of plant cells, tissues, and organs.

Editors and Affiliations

  • Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Fatima Cvrčková, Viktor Žárský

Bibliographic Information

  • Book Title: Plant Cell Morphogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Fatima Cvrčková, Viktor Žárský

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9469-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9468-7Published: 31 May 2019

  • Softcover ISBN: 978-1-4939-9471-7Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9469-4Published: 30 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 380

  • Number of Illustrations: 15 b/w illustrations, 53 illustrations in colour

  • Topics: Plant Physiology, Plant Sciences

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