Methods in Molecular Biology

Plant Innate Immunity

Methods and Protocols

Editors: Gassmann, Walter (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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About this book

The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource for all researchers interested in expanding their knowledge and learning new techniques in this ever-growing field.


Table of contents (22 chapters)

Table of contents (22 chapters)
  • Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic

    Pages 1-12

    Jayaraman, Jay (et al.)

  • Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine-Acetylated Proteins Expressed

    Pages 13-21

    Escouboué, Maxime (et al.)

  • Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl Coenzyme A

    Pages 23-32

    Schreiber, Karl J. (et al.)

  • A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions

    Pages 33-42

    Adhab, Mustafa (et al.)

  • Quantification of Extracellular ATP in Plant Suspension Cell Cultures

    Pages 43-54

    Ramachandran, Sowmya R. (et al.)

Buy this book

eBook $169.00
price for USA in USD
  • ISBN 978-1-4939-9458-8
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-4939-9457-1
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
Plant Innate Immunity
Book Subtitle
Methods and Protocols
Editors
  • Walter Gassmann
Series Title
Methods in Molecular Biology
Series Volume
1991
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9458-8
DOI
10.1007/978-1-4939-9458-8
Hardcover ISBN
978-1-4939-9457-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 260
Number of Illustrations
17 b/w illustrations, 42 illustrations in colour
Topics