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Plant Innate Immunity

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Walter Gassmann

    1. Division of Plant Sciences, Christopher S. Bond Life Sciences Center, and Interdisciplinary Plant Group, University of Missouri, Columbia, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1991)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Using Bioinformatics and Molecular Biology to Streamline Construction of Effector Libraries for Phytopathogenic Pseudomonas syringae Strains

    • Jay Jayaraman, Morgan K. Halane, Sera Choi, Honour C. McCann, Kee Hoon Sohn
    Pages 1-12

  3. Immunoprecipitation Under Non-Denaturing or Denaturing Conditions of Lysine-Acetylated Proteins Expressed in Planta

    • Maxime Escouboué, Laurent Deslandes
    Pages 13-21

  4. Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl Coenzyme A

    • Karl J. Schreiber, Jennifer D. Lewis
    Pages 23-32

  5. A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions

    • Mustafa Adhab, James E. Schoelz
    Pages 33-42

  6. Quantification of Extracellular ATP in Plant Suspension Cell Cultures

    • Sowmya R. Ramachandran, Sonika Kumar, Kiwamu Tanaka
    Pages 43-54

  7. Measuring Pectin Properties to Track Cell Wall Alterations During Plant–Pathogen Interactions

    • Gerit Bethke, Jane Glazebrook
    Pages 55-60

  8. Method to Study Dynamics of Membrane-Bound Plant Transcription Factors During Biotic Interactions in Tomato

    • Supriyo Chowdhury, Payel Bhattacharjee, Shrabani Basak, Shreya Chowdhury, Pallob Kundu
    Pages 61-68

  9. Preparation of Plant Material for Analysis of Protein–Nucleic Acid Interactions by FRET-FLIM

    • Maxime Escouboué, Laurent Camborde, Alain Jauneau, Elodie Gaulin, Laurent Deslandes
    Pages 69-77

  10. Analysis of DNA Methylation Profile in Plants by Chop-PCR

    • Pratiti Dasgupta, Shubho Chaudhuri
    Pages 79-90

  11. Change in Nucleosome Dynamics During Stress Responses in Plants

    • Amit Paul, Shubho Chaudhuri
    Pages 91-100

  12. Measuring Cell Ploidy Level in Arabidopsis thaliana by Flow Cytometry

    • Leiyun Yang, Zhixue Wang, Jian Hua
    Pages 101-106

  13. A Method for Investigating the Pseudomonas syringae-Arabidopsis thaliana Pathosystem Under Various Light Environments

    • Daniel L. Leuchtman, Anthony D. Shumate, Walter Gassmann, Emmanuel Liscum
    Pages 107-113

  14. Isolation and Characterization of Plant Metabolite Signals that Induce Type III Secretion by the Plant Pathogen Pseudomonas syringae

    • Conner J. Rogan, Jeffrey C. Anderson
    Pages 115-126

  15. Quantification of Cauline Leaf Abscission in Response to Plant Pathogens

    • O. Rahul Patharkar
    Pages 127-139

  16. Methods for Replicating Leaf Vibrations Induced by Insect Herbivores

    • Sabrina C. J. Michael, Heidi A. Appel, Reginald B. Cocroft
    Pages 141-157

  17. Infection Assay for Xanthomonas campestris pv. campestris in Arabidopsis thaliana Mimicking Natural Entry via Hydathodes

    • Marieke van Hulten, Sayantani Chatterjee, Harrold A. van den Burg
    Pages 159-185

  18. The Apple Fruitlet Model System for Fire Blight Disease

    • Sara M. Klee, Judith P. Sinn, Timothy W. McNellis
    Pages 187-198

  19. Generating Transgenic Arabidopsis Plants for Functional Analysis of Pathogen Effectors and Corresponding R Proteins

    • Sharon Pike, Walter Gassmann, Jianbin Su
    Pages 199-206

  20. Identification of Novel Pararetroviral Promoters for Designing Efficient Plant Gene Expression Systems

    • Ankita Shrestha, Ahamed Khan, Nrisingha Dey
    Pages 207-222
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Keywords

About this book

The volume presents valuable methods that look at important biological processes not traditionally assayed in the study of plant immunity, and at non-model systems. The chapters in this book cover topics such as identifying host targets of acetylating effectors by immunoprecipitation; quantifying ATP release from plant cells; protein-DNA interactions; DNA methylation; measurement and playback of leaf vibrations; natural infection routes of Xanthomonas campestris pv. campestris using Arabidopsis; and isolating favorable plant-growth-promoting bacteria from the phytosphere. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Plant Innate Immunity: Methods and Protocols is an essential resource forall researchers interested in expanding their knowledge and learning new techniques in this ever-growing field.


Editors and Affiliations

  • Division of Plant Sciences, Christopher S. Bond Life Sciences Center, and Interdisciplinary Plant Group, University of Missouri, Columbia, USA

    Walter Gassmann

Bibliographic Information

  • Book Title: Plant Innate Immunity

  • Book Subtitle: Methods and Protocols

  • Editors: Walter Gassmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9458-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9457-1Published: 30 April 2019

  • eBook ISBN: 978-1-4939-9458-8Published: 30 April 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 260

  • Number of Illustrations: 17 b/w illustrations, 42 illustrations in colour

  • Topics: Plant Sciences

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