Methods in Molecular Biology

Antigen Processing

Methods and Protocols

Editors: van Endert, Peter (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $109.00
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  • ISBN 978-1-4939-9450-2
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Hardcover $219.99
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About this book

The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approaches to measure processing efficacy; description of different assays measuring MHC recycling; and protocols to produce MHC class II tetramers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Antigen Processing: Methods and Protocols, Second Edition is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field.

Table of contents (30 chapters)

Table of contents (30 chapters)
  • Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes

    Pages 1-14

    Tenzer, Stefan (et al.)

  • Analysis of Proteasome-Generated Antigenic Peptides by Mass Spectrometry

    Pages 15-29

    Textoris-Taube, Kathrin (et al.)

  • Trimming of MHC Class I Ligands by ERAP Aminopeptidases

    Pages 31-43

    Weimershaus, Mirjana (et al.)

  • Peptide Trimming for MHC Class I Presentation by Endoplasmic Reticulum Aminopeptidases

    Pages 45-57

    Compagnone, Mirco (et al.)

  • Testing the Impact of Protease Inhibitors in Antigen Presentation Assays

    Pages 59-69

    Basler, Michael (et al.)

Buy this book

eBook $109.00
price for USA in USD
  • ISBN 978-1-4939-9450-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • Immediate eBook download after purchase and usable on all devices
  • Bulk discounts available
Hardcover $219.99
price for USA in USD
Softcover $149.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Antigen Processing
Book Subtitle
Methods and Protocols
Editors
  • Peter van Endert
Series Title
Methods in Molecular Biology
Series Volume
1988
Copyright
2019
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-4939-9450-2
DOI
10.1007/978-1-4939-9450-2
Hardcover ISBN
978-1-4939-9449-6
Softcover ISBN
978-1-4939-9452-6
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XV, 458
Number of Illustrations
34 b/w illustrations, 45 illustrations in colour
Topics