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Antigen Processing

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1988)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
  2. Analysis of Proteasome-Generated Antigenic Peptides by Mass Spectrometry

    • Kathrin Textoris-Taube, Ulrike Kuckelkorn, Christin Beier, Peter M. Kloetzel
    Pages 15-29
  3. Trimming of MHC Class I Ligands by ERAP Aminopeptidases

    • Mirjana Weimershaus, Irini Evnouchidou, Lenong Li, Peter van Endert, Marlene Bouvier
    Pages 31-43
  4. Measuring Synthesis and Degradation of MHC Class I Molecules

    • Simon J. Powis, Antony N. Antoniou
    Pages 83-100
  5. A SIINFEKL-Based System to Measure MHC Class I Antigen Presentation Efficiency and Kinetics

    • Devin Dersh, Jonathan W. Yewdell, Jiajie Wei
    Pages 109-122
  6. Purification and Identification of Naturally Presented MHC Class I and II Ligands

    • Annika Nelde, Daniel J. Kowalewski, Stefan Stevanović
    Pages 123-136
  7. Identification of MHC Ligands and Establishing MHC Class I Peptide Motifs

    • Michael Ghosh, Moreno Di Marco, Stefan Stevanović
    Pages 137-147
  8. Identifying Antigens Recognized by Cytolytic T Lymphocytes on Tumors

    • Nathalie Vigneron, Vincent Stroobant, Wenbin Ma, Alexandre Michaux, Benoît J. Van den Eynde
    Pages 159-186
  9. Evaluating CD8+ T Cell Responses In Vitro

    • François A. Lemonnier
    Pages 199-215
  10. Recombinant Poxviruses: Versatile Tools for Immunological Assays

    • Katherine S. Forsyth, Mary M. Addison, Laurence C. Eisenlohr
    Pages 217-248
  11. Monitoring Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy

    • Maria Embgenbroich, Jasmin Mertins, Thorsten Lang, Sven Burgdorf
    Pages 249-257

About this book

The second edition volume expands on the previous edition with new and updated chapters on the latest methods used for studying presentation of antigenic peptides produced in the standard processing pathways for MHC class I and II molecules. The new chapters cover topics such as biochemical and cellular approaches to study the impact of the endoplasmic reticulum aminopeptidases; techniques to monitor MHC class I synthesis and degradation; approaches to measure processing efficacy; description of different assays measuring MHC recycling; and protocols to produce MHC class II tetramers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and comprehensive, Antigen Processing: Methods and Protocols, Second Edition is a valuable tool for both novice and expert researchers interested in studying antigen processing and venturing out further into this evolving field.


Editors and Affiliations

  • INSERM U1151, Université Paris Descartes, Hopital Necker-Enfants Malades, Paris, France

    Peter van Endert

Bibliographic Information

  • Book Title: Antigen Processing

  • Book Subtitle: Methods and Protocols

  • Editors: Peter van Endert

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9450-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9449-6Published: 30 May 2019

  • Softcover ISBN: 978-1-4939-9452-6Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9450-2Published: 30 May 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 458

  • Number of Illustrations: 34 b/w illustrations, 45 illustrations in colour

  • Topics: Molecular Medicine, Antibodies

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access