TRP Channels

1. Front Matter

   Pages i-x

   PDF

2. Molecular Evolution Bioinformatics Toward Structural Biology of TRPV1-4 Channels

   • Pau Doñate-Macián, Alba Crespi-Boixader, Alex Perálvarez-Marín

   Pages 1-21

3. Expression, Purification, and Crystallization of the Transient Receptor Potential Channel TRPV6

   • Appu K. Singh, Luke L. McGoldrick, Alexander I. Sobolevsky

   Pages 23-37

4. Cryo Electron Microscopy of TRP Channels

   • Amrita Samanta, Taylor E. T. Hughes, Vera Y. Moiseenkova-Bell

   Pages 39-50

5. Mass Spectrometry-Based Analysis of TRPP2 Phosphorylation

   • Verónica I. Dumit, Michael Köttgen, Alexis Hofherr

   Pages 51-64

6. Combining Structural Data with Computational Methodologies to Investigate Structure–Function Relationships in TRP Channels

   • Victoria Oakes, Carmen Domene

   Pages 65-82

7. Characterization of TRPC Channels in a Heterologous System Using Calcium Imaging and the Patch-Clamp Technique

   • Elvira de la Peña, Ana Gomis

   Pages 83-97

8. Fluorescence-Based Functional Assays for Ca2+-Permeable ThermoTRP Channels

   • Celia Cordero-Sánchez, Irene Mudarra-Fraguas, Asia Fernández-Carvajal

   Pages 99-110

9. High-Content Imaging of Immunofluorescently Labeled TRPV1-Positive Sensory Neurons

   • Jörg Isensee, Tim Hucho

   Pages 111-124

10. Patch-Clamp Combined with Fast Temperature Jumps to Study Thermal TRP Channels

    • Beiying Liu, Feng Qin

    Pages 125-141

11. TRP Channel Reconstitution in Lipid Bilayers

    • Eleonora Zakharian

    Pages 143-166

12. Methods for Investigating TRP Channel Gating

    • Osvaldo Alvarez, Karen Castillo, Emerson Carmona, Carlos Gonzalez, Ramon Latorre

    Pages 167-185

13. In Silico Approaches for TRP Channel Modulation

    • Magdalena Nikolaeva Koleva, Gregorio Fernandez-Ballester

    Pages 187-206

14. Drug Discovery for Soft Drugs on TRPV1 and TRPM8 Channels Using the Passerini Reaction

    • Tracey Pirali, Ubaldina Galli, Marta Serafini, Alessia Griglio, Armando A. Genazzani, Gian Cesare Tron

    Pages 207-221

15. In Vivo Methods to Study ThermoTRP Channels in Rodents

    • Sara González-Rodríguez

    Pages 223-231

16. Back Matter

    Pages 233-238

    PDF

This book aims to provide a guide to researchers on how to work with Transient Receptor Potential (TRP) channels, describing current methodologies and protocols, and highlighting the challenges ahead that will require the development of new technologies. Articles cover a wide range of techniques, from structure to function, concluding with how to best to evaluate some of the in vivo function of TRP channels, particularly in regard to their involvement in hyperalgesia and allodynia. Also, the collection describes important and inspiring available tools that are currently being used to investigate the activity of these channels in vitro and in vivo in terms of their underlying protein structure, segueing into dealing with the generation of pharmacological tools to explore their in vivo activity and their involvement in cellular signaling pathways, which may be developed into drugs to treat diseases caused by channel dysfunction or hyperactivity. Written for the highly successful Methods in Molecular Biology series, chapters include the necessary detail and implementation advice to ensure successful results in the lab. 

Authoritative and practical, TRP Channels: Methods and Protocols is a useful set of methodologies for scientists seeking to better understand this vital family of channels.

• Transient Receptor Potential channels
• Non-selective cationic channels
• Protein structure
• Pharmacology
• Therapeutics
• Cellular signaling pathways
• Channel dysfunction

• Instituto de Biología, Molecular y Celular, Universidad Miguel Hernández, Elche, Spain

  Antonio Ferrer-Montiel

• Experimental Anesthesiology and Pain Research, Department of Anesthesiology and Intensive Care Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany

  Tim Hucho

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